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Clathrin, Light Chain (AA 156-173) 抗体

Cited in 11+ publications. This anti-Clathrin, Light Chain antibody is a 小鼠 单克隆 antibody detecting Clathrin, Light Chain in IHC, WB, ICC 和 IHC (p). Suitable for 人, 小鼠, 大鼠 和 斑马鱼.
产品编号 ABIN1742245
发货至: 中国
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Quick Overview for Clathrin, Light Chain (AA 156-173) 抗体 (ABIN1742245)

抗原

Clathrin, Light Chain

适用

人, 小鼠, 大鼠, 斑马鱼

宿主

  • 10
  • 1
  • 1
小鼠

克隆类型

  • 10
  • 2
单克隆

标记

  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
非结合性

应用范围

  • 12
  • 11
  • 9
  • 9
  • 1
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

57-4
  • 抗原表位

    • 10
    • 1
    AA 156-173

    原理

    Mouse anti-Clathrin light chain IgG

    特异性

    Specific for both neuronal light chains

    交叉反应 (详细)

    Reacts with: human (P09497), rat (P08082), mouse (Q6IRU5), zebrafish. Other species not tested yet.

    纯化方法

    purified IgG

    免疫原

    Synthetic peptide corresponding to AA 156 to 173 from rat Clathrin light chainB (UniProt Id: P08082)

    亚型

    IgG1
  • 应用备注

    WB:1 : 5000 up to 1 : 10000 , IP:not tested yet ICC:1 : 500 up to 1 : 1000, IHC:1 : 500, IHC-P:1 : 2000,

    说明

    Suitable for immunogold electron microscopy.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

    缓冲液

    PBS. Albumin and azide were added for stabilization.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

    储存条件

    -20 °C,-80 °C,4 °C

    储存方法

    Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!
  • Neef, Jung, Wong, Reuter, Pangrsic, Chakrabarti, Kügler, Lenz, Nouvian, Boumil, Frankel, Wichmann, Moser: "Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 3, pp. 705-16, (2014) (PubMed).

    Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

    Biesemann, Grønborg, Luquet, Wichert, Bernard, Bungers, Cooper, Varoqueaux, Li, Byrne, Urlaub, Jahn, Brose, Herzog: "Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting." in: The EMBO journal, Vol. 33, Issue 2, pp. 157-70, (2014) (PubMed).

    Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Hellwig, Hack, Kowalski, Brunne, Jarowyj, Unger, Bock, Junghans, Frotscher: "Role for Reelin in neurotransmitter release." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 7, pp. 2352-60, (2011) (PubMed).

    Ferguson, Brasnjo, Hayashi, Wölfel, Collesi, Giovedi, Raimondi, Gong, Ariel, Paradise, Otoole, Flavell, Cremona, Miesenböck, Ryan, De Camilli: "A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis." in: Science (New York, N.Y.), Vol. 316, Issue 5824, pp. 570-4, (2007) (PubMed).

    Krauss, Kukhtina, Pechstein, Haucke: "Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2mu-cargo complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 32, pp. 11934-9, (2006) (PubMed).

    Johnson, Brown, Pulliam, Anderson, Johnson: "Synaptic pathology, altered gene expression, and degeneration in photoreceptors impacted by drusen." in: Investigative ophthalmology & visual science, Vol. 46, Issue 12, pp. 4788-95, (2005) (PubMed).

    von Kriegstein, Schmitz: "The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina." in: Cell and tissue research, Vol. 311, Issue 2, pp. 159-73, (2003) (PubMed).

    Blumstein, Faundez, Nakatsu, Saito, Ohno, Kelly: "The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 20, pp. 8034-42, (2001) (PubMed).

    Von Kriegstein, Schmitz, Link, Südhof: "Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses." in: The European journal of neuroscience, Vol. 11, Issue 4, pp. 1335-48, (1999) (PubMed).

  • 抗原

    Clathrin, Light Chain

    别名

    Clathrin light chain

    背景

    Synonyms: Lcb, Cltb

    Antigen Information: Clathrin light chain is a small clathrin coat subunit involved in endocytosis

    UniProt

    P08082
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