SNAP alpha/beta 抗体
This anti-SNAP alpha/beta antibody is a 小鼠 单克隆 antibody detecting SNAP alpha/beta in WB, ICC 和 IP. Suitable for 人, 小鼠, 大鼠 和 斑马鱼. This Primary Antibody has been cited in 3+ publications.
(3 references)Quick Overview for SNAP alpha/beta 抗体 (ABIN1742242)
抗原
SNAP alpha/beta
适用
人, 小鼠, 大鼠, 斑马鱼
宿主
小鼠
克隆类型
单克隆
标记
This SNAP alpha/beta antibody is un-conjugated
应用范围
Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
克隆位点
77-2
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特异性
- Specific for alpha- and beta SNAP
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交叉反应 （详细）
- does not cross-react to gamma SNAP.
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纯化方法
- purified IgG
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免疫原
- Recombinant full length rat alpha SNAP.
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亚型
- IgG1
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anti-SNAP alpha/beta antibody
适用: 人, 小鼠, 大鼠, 斑马鱼 WB 宿主: 小鼠 Monoclonal 77-1 unconjugated
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应用备注
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WB: 1 : 1000 up to 1 : 10000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC: not tested yet -
说明
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The antibody does not immunoprecipitate the 20 S SNARE-complex.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
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缓冲液
- PBS
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注意事项
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
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储存条件
- -20 °C
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储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).
: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).
: "The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin." in: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16955-61, (1995) (PubMed).
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: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).
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- SNAP alpha/beta
抗原
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