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SNAP alpha/beta 抗体 (AA 1-295)

Cited in 3+ publications. This 小鼠 单克隆 anti-SNAP alpha/beta antibody (Clone 77-2) specifically detects SNAP alpha/beta in WB, ICC 和 IP. The antibody is reactive with 人, 小鼠, 大鼠 和 斑马鱼 samples.
产品编号 ABIN1742242
发货至: 中国
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Quick Overview for SNAP alpha/beta 抗体 (AA 1-295) (ABIN1742242)

抗原

SNAP alpha/beta

适用

人, 小鼠, 大鼠, 斑马鱼

宿主

  • 1
  • 1
小鼠

克隆类型

  • 1
  • 1
单克隆

标记

  • 2
This SNAP alpha/beta antibody is un-conjugated

应用范围

Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

克隆位点

77-2
  • 抗原表位

    AA 1-295

    原理

    Mouse anti-alpha/beta SNAP IgG

    特异性

    Specific for alpha- and beta SNAP

    交叉反应 (详细)

    Reacts with: human (P54920, P60880), rat (P54921, P60881), mouse (Q9DB05, P28663), zebrafish. Other species not tested yet.

    纯化方法

    purified IgG

    免疫原

    Recombinant protein corresponding to AA 1 to 295 from rat alpha SNAP (UniProt Id: P54921)

    亚型

    IgG1
  • 应用备注

    WB:1 : 1000 up to 1 : 10000 IHC-P:not tested yet

    说明

    The antibody does not immunoprecipitate the 20 S SNARE-complex.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

    缓冲液

    PBS.

    注意事项

    Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

    储存条件

    -20 °C,-80 °C,4 °C

    储存方法

    Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Do not freeze!
  • de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).

    Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Hanson, Otto, Barton, Jahn: "The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin." in: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16955-61, (1995) (PubMed).

  • 抗原

    SNAP alpha/beta

    背景

    Synonyms: SNARE associated, alpha SNAP, beta SNAP, alpha-SNAP, beta-SNAP

    Antigen Information: alpha/beta SNAP are peripheral membrane proteins required for membrane traffic

    UniProt

    P54921
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