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SNAP25 抗体 (AA 192-206)

Name: SNAP25 抗体 (AA 192-206)
SKU: ABIN1742235
Availability: OutOfStock

This anti-SNAP25 antibody is a 兔多克隆 antibody detecting SNAP25 in WB, ELISA, IHC, IP 和 ICC. Suitable for 人, 大鼠, 小鼠, 小鸡, 斑马鱼, Hamster 和 Goldfish. This Primary Antibody has been cited in 9+ publications.

$dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2) (SNAP25 抗体 (AA 192-206))$ WB

Indirect immunostaining of PFA fixed rat hippocampus neurons (dilution 1 : 500; green). (SNAP25 抗体 (AA 192-206))

ICC

Indirect immunofluorescence labeling of PFA fixed, paraffin embedded mouse cerebellum section with anti-SNAP 25 (dilution 1 : 500; red) and mouse anti-calbindin (cat. (SNAP25 抗体 (AA 192-206))

IHC (p)

Indirect immunostaining of a PFA fixed mouse hippocampus section with anti-SNAP 25 (dilution 1 : 200; red) and guinea pig anti-VGluT 2 (cat. (SNAP25 抗体 (AA 192-206))

IHC

4 images

(9 references)

产品编号 ABIN1742235

发货至: 中国

Datasheet as PDF

Quick Overview for SNAP25 抗体 (AA 192-206) (ABIN1742235)

抗原

See all SNAP25 抗体

SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

适用

人, 大鼠, 小鼠, 小鸡, 斑马鱼, Hamster, Goldfish

宿主

兔

克隆类型

多克隆

标记

This SNAP25 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Discover our top product SNAP25 Primary Antibody

抗原表位
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15

12

8

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3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 192-206

特异性

Specific for SNAP 25.

纯化方法

antiserum

免疫原

Synthetic peptide IDEANQRATKMLGSG (aa 192-206 in human SNAP 25) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 1-206) antibody
SNAP25 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 1-206) antibody
SNAP25 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (C-Term) antibody
Verified SNAP25 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) antibody
SNAP25 适用: 人 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 1E6 unconjugated

anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 166-206) antibody
SNAP25 适用: 大鼠, 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) antibody
SNAP25 适用: 人 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 1G2 unconjugated

anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 31-130) antibody
SNAP25 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4A3 unconjugated

anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (C-Term) antibody
SNAP25 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) antibody
SNAP25 适用: 人, 大鼠, 小鼠, Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) antibody
SNAP25 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1 : 10000 (AP staining)
ICC: 1 : 100

说明

Recognizes the Botulinum neurotoxin A cleavage product with reduced affinity. Does not detect the neurotoxin E cleavage product. Recognizes splice variants SNAP 25A and B. ELISA: Suitable as detector antibody for sandwich-ELISA as capture antibody (protocol for sandwich-ELISA).

限制

仅限研究用
状态

Liquid

缓冲液

PBS

注意事项

Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

储存条件

4 °C/-20 °C

储存方法

Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Schafer, Heller, Gunner, Heller, Gordon, Hammond, Wolf, Jung, Stevens: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." in: eLife, Vol. 5, (2016) (PubMed).

Fossati, Morini, Corradini, Antonucci, Trepte, Edry, Sharma, Papale, Pozzi, Defilippi, Meier, Brambilla, Turco, Rosenblum, Wanker, Ziv, Menna, Matteoli: "Reduced SNAP-25 increases PSD-95 mobility and impairs spine morphogenesis." in: Cell death and differentiation, Vol. 22, Issue 9, pp. 1425-36, (2015) (PubMed).

Walter, Kurps, de Wit, Schöning, Toft-Bertelsen, Lauks, Ziomkiewicz, Weiss, Schulz, Fischer von Mollard, Verhage, Sørensen: "The SNARE protein vti1a functions in dense-core vesicle biogenesis." in: The EMBO journal, Vol. 33, Issue 15, pp. 1681-97, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Kung, Gong, Adedoyin, Ng, Bhargava, Ohara, Jasmin: "Evidence for glutamate as a neuroglial transmitter within sensory ganglia." in: PLoS ONE, Vol. 8, Issue 7, pp. e68312, (2013) (PubMed).

Young, Franciosi, Spreeuw, Deng, Sanders, Tam, Huang, Singaraja, Zhang, Bissada, Kay, Hayden: "Low levels of human HIP14 are sufficient to rescue neuropathological, behavioural, and enzymatic defects due to loss of murine HIP14 in Hip14-/- mice." in: PLoS ONE, Vol. 7, Issue 5, pp. e36315, (2012) (PubMed).

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Camoletto, Vara, Morando, Connell, Marletto, Giustetto, Sassoè-Pognetto, Van Veldhoven, Ledesma: "Synaptic vesicle docking: sphingosine regulates syntaxin1 interaction with Munc18." in: PLoS ONE, Vol. 4, Issue 4, pp. e5310, (2009) (PubMed).

Johansson, Ericsson, Janson, Beraki, Stanić, Mandic, Wikström, Hökfelt, Ogren, Rozell, Berggren, Bark: "An ancient duplication of exon 5 in the Snap25 gene is required for complex neuronal development/function." in: PLoS genetics, Vol. 4, Issue 11, pp. e1000278, (2008) (PubMed).
抗原

SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

别名

SNAP 25

途径

Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport