STX8 抗体 (Cytoplasmic Domain)
Quick Overview for STX8 抗体 (Cytoplasmic Domain) (ABIN1742226)
抗原
See all STX8 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-215, Cytoplasmic Domain
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特异性
- Specific for syntaxin 8.
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纯化方法
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
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免疫原
- Recombinant cytoplasmic domain of rat syntaxin 8 (aa 1-215).
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应用备注
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WB: 1 : 100 up to 1 : 5000 (AP staining)
ICC: 1 : 100 up to 1 : 500
IHC: not tested yet -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
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缓冲液
- PBS
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注意事项
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
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储存条件
- -20 °C
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储存方法
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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: "SNARE protein expression and localization in human cytotoxic T lymphocytes." in: European journal of immunology, Vol. 42, Issue 2, pp. 470-5, (2012) (PubMed).
: "Salmonella acquires lysosome-associated membrane protein 1 (LAMP1) on phagosomes from Golgi via SipC protein-mediated recruitment of host Syntaxin6." in: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5574-87, (2012) (PubMed).
: "Syntaxin 11 binds Vti1b and regulates late endosome to lysosome fusion in macrophages." in: Traffic (Copenhagen, Denmark), Vol. 12, Issue 6, pp. 762-73, (2011) (PubMed).
: "A VAMP7/Vti1a SNARE complex distinguishes a non-conventional traffic route to the cell surface used by KChIP1 and Kv4 potassium channels." in: The Biochemical journal, Vol. 418, Issue 3, pp. 529-40, (2009) (PubMed).
: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).
: "Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis Factor-alpha." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10478-83, (2005) (PubMed).
: "MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking." in: Molecular biology of the cell, Vol. 16, Issue 4, pp. 1696-710, (2005) (PubMed).
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: "SNARE protein expression and localization in human cytotoxic T lymphocytes." in: European journal of immunology, Vol. 42, Issue 2, pp. 470-5, (2012) (PubMed).
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- STX8 (Syntaxin 8 (STX8))
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别名
- Syntaxin 8
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途径
- Synaptic Vesicle Exocytosis
抗原
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