RAB3A 抗体 (AA 154-227)
Quick Overview for RAB3A 抗体 (AA 154-227) (ABIN1742210)
抗原
See all RAB3A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 154-227
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特异性
- Specific for all four Rab 3 isoforms a, b, c, d.
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纯化方法
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
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免疫原
- Recombinant human Rab 3c (aa 154-227).
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应用备注
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WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 1000
IHC: 1 : 200 up to 1 : 500 -
说明
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This antibody has been affinity purified with the complete antigen (aa 154 - 227) and recognizes all isoforms, due to the homology of Rab 3 proteins.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
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缓冲液
- PBS
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注意事项
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
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储存条件
- -20 °C
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储存方法
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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: "A secretagogin locus of the mammalian hypothalamus controls stress hormone release." in: The EMBO journal, Vol. 34, Issue 1, pp. 36-54, (2015) (PubMed).
: "Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission." in: The Journal of cell biology, Vol. 201, Issue 6, pp. 915-28, (2013) (PubMed).
: "Synaptotagmin-1 docks secretory vesicles to syntaxin-1/SNAP-25 acceptor complexes." in: Cell, Vol. 138, Issue 5, pp. 935-46, (2009) (PubMed).
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: "A secretagogin locus of the mammalian hypothalamus controls stress hormone release." in: The EMBO journal, Vol. 34, Issue 1, pp. 36-54, (2015) (PubMed).
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- RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))
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别名
- Rab 3
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途径
- Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
抗原
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