LYZ 抗体
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北京 101111
Quick Overview for LYZ 抗体 (ABIN1741599)
抗原
See all LYZ 抗体适用
宿主
克隆类型
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应用范围
克隆位点
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原理
- This product is optimised for use with FIX&PERM®.
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特异性
- The anti-Lysozyme Antibody (clone LZ-2) reacts with intracellular human lysozyme/muramidase expressed by virtually all myelomonocytic cells, macrophages and their precursors. The sensitivity of LZ-2 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells).In practice, 50 µL of leukocytes containing 10^7 cells/mL are stained with 20 µL mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.
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产品特性
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Mildly fixes cells, preserving their flow cytometric scatter characteristics
Allows simultaneous characterisation of both intracellular and cell surface markers
Rapid technique - whole procedure can be carried out in less than one hour, ready
for immediate analysis or storage for 24 hours
Stringent QC procedures - the quality of each lot is determined using well-defined
blood samples and subsequent comparison of scatter characteristics of obtained
leukocyte populations, ensuring consistent and reliable results lot after lot
A range of intracellular antibodies with optimised protocols for use with FIX&PERM®
FIX&PERM® is a simple procedure making use of two reagents. Reagent A gently fixes cells, while Reagent B permeabilizes them. The specific formulations reduce background and allow simultaneous addition of permeabilization medium and fluorochrome labelled antibodies, allowing staining of intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins, etc. -
纯化方法
- Purified by Affinity Chromatography
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亚型
- IgG1
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anti-Lysozyme (LYZ) (AA 1-100) antibody
LYZ 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 19-148) antibodyLYZ 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) antibodyLYZ 适用: 人 ICC, FACS, MS 宿主: 小鼠 Monoclonal LZ598-10G9 unconjugated
anti-Lysozyme (LYZ) antibodyLYZ 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IHC (fro), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 24-144) antibodyLYZ 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 22-143) antibodyLYZ 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 19-148) antibodyLYZ 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 18-147) antibodyLYZ 适用: Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) antibody (FITC)LYZ 适用: 人 ICFC 宿主: 小鼠 Monoclonal LZ598-10G9 FITC
anti-Lysozyme (LYZ) antibodyLYZ 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1C9 unconjugated
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应用备注
- Permeabilization and Staining Procedure - In combination with our Permeabilization Kit FIX&PERM® (ABIN1741575) intracellular Lysozyme can be easily stained in cell suspensions. - For each sample to be analyzed add 50 µL of whole blood, bone marrow or mononuclear cell suspension in a 5 mL tube - Add 100 µL of Reagent A (Fixation Medium, stored and used at room temperature) - Incubate for 15 minutes at room temperature - Add 5 mL phosphate buffered saline and centrifuge cells for 5 minutes at 300 g - Remove supernatant and add to cell pellet 100 µL Reagent B (Permeabilization Medium) and 20 µL of the LZ-2 monoclonal antibody conjugate - Vortex at low speed for 1-2 seconds - Incubate for 15 minutes at room temperature - Wash cells with phosphate buffered saline as described above - Remove supernatant and resuspend cells in sheath fluid for immediate analysis or resuspend cells in 0.5 mL 1.0 % formaldehyde and store them at 2- 8°C in the dark. Analyze fixed cells within 24 hours.
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说明
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Lysozyme (LZ) is a cationic antimicrobial peptide of 14 kDa. Lysozyme is stored in primary but predominantly in specific (secondary) granules of neutrophils. It cleaves peptidoglycan constituents of the bacterial cell wall and can bind LPS. The epitope recognized by antibody LZ-2 is expressed by virtually all myeloid cells including normal and malignant granulocytes and monocytes. In normal myelopoiesis LZ can first be detected at the myeloblast stage where it appears somewhat later than MPO expression. The LZ-2 antibody permits the identification and enumeration of human myelomonocytic cells using flow cytometry. Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls.
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实验流程
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Procedure: For each sample to be analysed use 50 µL of whole blood, bone marrow or mononuclear cell suspension in a 5 mL tube
Add 100 µL of Reagent A (Fixation Medium, stored and used at room temperature)
Incubate for 15 minutes at room temperature
Add 5 mL phosphate buffered saline and centrifuge cells for 5 minutes at 300 g
Remove supernatant and add to cell pellet 100 µL Reagent B (Permeabilization
Medium) and 20 µL of the appropriate monoclonal antibody conjugate
Vortex at low speed for 1-2 seconds
Incubate for 15 minutes at room temperature
Wash cells with phosphate buffered saline as described above
Remove supernatant and re-suspend cells in sheath fluid for immediate analysis or re-suspend cells in 0.5 mL 1.0 % formaldehyde and store at 2-8°C in the dark
Analyse fixed cells within 24 hours -
限制
- 仅限研究用
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缓冲液
- PBS pH 7.2, 1 % BSA, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This reagent contains sodium azide. To avoid the development of hazardous conditions, reagents containing azide should be diluted in running water prior to be discarded. Similar to the work with other biological products, proper handling procedures are recommended.
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注意事项
- Do not freeze and protect from prolonged exposure to light.
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储存条件
- 4 °C
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储存方法
- These reagents should be stored at 2-8°C Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.
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: "Myeloid cell-associated lysosomal proteins as flow cytometry markers for leukocyte lineage classification." in: Journal of biological regulators and homeostatic agents, Vol. 18, Issue 3-4, pp. 335-9, (2005) (PubMed).
: "Optimal number of reagents required to evaluate hematolymphoid neoplasias: results of an international consensus meeting." in: Cytometry, Vol. 46, Issue 1, pp. 23-7, (2001) (PubMed).
: "The individual regulation of granule protein mRNA levels during neutrophil maturation explains the heterogeneity of neutrophil granules." in: Journal of leukocyte biology, Vol. 66, Issue 6, pp. 989-95, (2000) (PubMed).
: "Intracellular markers in acute myeloid leukemia diagnosis." in: Neoplasma, Vol. 45, Issue 5, pp. 282-91, (1999) (PubMed).
: "Neutrophil granulocyte-committed cells can be driven to acquire dendritic cell characteristics." in: The Journal of experimental medicine, Vol. 187, Issue 7, pp. 1019-28, (1998) (PubMed).
: "Comparative analysis of different permeabilization methods for the flow cytometry measurement of cytoplasmic myeloperoxidase and lysozyme in normal and leukemic cells." in: Cytometry, Vol. 30, Issue 3, pp. 134-44, (1997) (PubMed).
: "Flow cytometric detection of intracellular antigens for immunophenotyping of normal and malignant leukocytes." in: Leukemia, Vol. 10, Issue 8, pp. 1383-9, (1996) (PubMed).
: "A classification of acute leukaemia for the 1990s." in: Annals of hematology, Vol. 62, Issue 1, pp. 16-21, (1991) (PubMed).
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: "Myeloid cell-associated lysosomal proteins as flow cytometry markers for leukocyte lineage classification." in: Journal of biological regulators and homeostatic agents, Vol. 18, Issue 3-4, pp. 335-9, (2005) (PubMed).
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- LYZ (Lysozyme (LYZ))
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别名
- Lysozyme
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分子量
- 14 kDa
抗原
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