KCNK3 抗体 (AA 251-411)
Quick Overview for KCNK3 抗体 (AA 251-411) (ABIN1741485)
抗原
See all KCNK3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 251-411
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特异性
- Detects ~50 kDa. Does not cross-react with TASK3.
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Protein G Purified
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免疫原
- Fusion protein amino acids 251-411 (cytoplasmic C-terminus) of rat Acid-sensitive potassium channel protein TASK or TASK1. Mouse: 96% identity (156/161 amino acids identical). Human: 76% identity (163/161 amino acids identical). <30% identity with TASK3.
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亚型
- IgG2b
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应用备注
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
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说明
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1 μg/ml of ABIN1741485 was sufficient for detection of TASK1 Potassium Channel in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- -20°C
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- KCNK3 (Potassium Channel, Subfamily K, Member 3 (KCNK3))
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别名
- KCNK3
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背景
- K+ channels are divided into three subclasses reflecting the number of transmembrane segments (TMS), which are designated 6TMS, 4TMS and 2TMS. Members of the 4TMS class contain two distinct pore regions and include TWIK, TREK, TRAAK and TASK. TASK channels are highly sensitive to external pH in the physiological range. TASK-1 is expressed in brain and in rat heart, with high levels of expression in the right atrium. TASK-2, mainly expressed in kidney, is localized in cortical distal tubules and collecting ducts, suggesting a role in renal K+ transport. TASK-3 from rat cerebellum shares 54 % identity with TASK-1, but less than 30 % identity with TASK-2 and other tandem pore K+ channels.
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基因ID
- 29553
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NCBI登录号
- NP_203694
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UniProt
- O54912
抗原
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