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GFAP 抗体 (AA 411-422) (Biotin)

This Biotin-conjugated 小鼠 单克隆 anti-GFAP antibody (Clone S206A-8) specifically detects GFAP in WB, IHC, IF 和 ICC. The antibody is reactive with 人 samples.
产品编号 ABIN1740956
发货至: 中国
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Quick Overview for GFAP 抗体 (AA 411-422) (Biotin) (ABIN1740956)

抗原

See all GFAP 抗体
GFAP (Glial Fibrillary Acidic Protein (GFAP))

适用

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小鼠

克隆类型

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单克隆

标记

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This GFAP antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

克隆位点

S206A-8
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    AA 411-422

    特异性

    Detects ~50 kDa. Cross-reacts with GFAP-R416W and other GFAP mutant proteins.

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Protein G Purified

    免疫原

    Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP, 100% identical in rat and mouse

    亚型

    IgG1
  • 应用备注

    • WB (1:1000)
    • optimal dilutions for assays should be determined by the user.

    说明

    1 μg/ml of ABIN1740956 was sufficient for detection of GFAP in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Conjugated antibodies should be stored at 4°C
  • 抗原

    GFAP (Glial Fibrillary Acidic Protein (GFAP))

    别名

    GFAP

    背景

    The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia.

    基因ID

    2670

    NCBI登录号

    NP_001124491

    UniProt

    P14136
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