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CD22 抗体 (AA 621-725)

Name: CD22 抗体 (AA 621-725)
Brand: antibodies-online
SKU: ABIN1724888
Availability: OutOfStock

This anti-CD22 antibody is a 小鼠单克隆 antibody detecting CD22 in FACS, WB, ELISA, IHC 和 ICC. Suitable for 人. This Primary Antibody has been cited in 2+ publications.

Flow cytometric analysis of Hela cells using CD22 mouse mAb (green) and negative control (red). (CD22 抗体 (AA 621-725))

FACS

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (CD22 抗体 (AA 621-725))

ELISA

Immunofluorescence analysis of Hela cells using CD22 mouse mAb (green). (CD22 抗体 (AA 621-725))

Western blot analysis using CD22 mouse mAb against L1210 (1), Hela (2), HEK293 (3), Jurkat (4), OCM-1 (5), A432 (6) and NIH/3T3 (7) cell lysate. (CD22 抗体 (AA 621-725))

Western blot analysis using CD22 mAb against human CD22 recombinant protein. (CD22 抗体 (AA 621-725))

Western blot analysis using CD22 mAb against HEK293 (1) and CD22 (AA: 621-725)-hIgGFc transfected HEK293 (2) cell lysate. (CD22 抗体 (AA 621-725))

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ZEB1 mouse mAb with DAB staining. (CD22 抗体 (AA 621-725))

IHC

Immunohistochemical analysis of paraffin-embedded cerebellum tissues using ZEB1 mouse mAb with DAB staining. (CD22 抗体 (AA 621-725))

IHC

8 images

(2 references)

产品编号 ABIN1724888

发货至: 中国

Quick Overview for CD22 抗体 (AA 621-725) (ABIN1724888)

抗原

See all CD22 抗体

CD22 (CD22 Molecule (CD22))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This CD22 antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

克隆位点

2H1C4

抗原表位
27

11

9

8

4

4

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 621-725

原理

CD22 Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human CD22 (AA: 621-725) expressed in E. Coli.

亚型

IgG1
anti-CD22 Molecule (CD22) (AA 52-178) antibody
CD22 适用: 人 FACS, WB, ELISA, IHC, IF, Coat, StM 宿主: 小鼠 Monoclonal BLCAM-1795 unconjugated

anti-CD22 Molecule (CD22) (AA 621-725) antibody
CD22 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 1A3A11 unconjugated

anti-CD22 Molecule (CD22) (AA 52-178) antibody
CD22 适用: 人 FACS, WB, ELISA, IHC, IF, Coat 宿主: 小鼠 Monoclonal BLCAM-1796 unconjugated

anti-CD22 Molecule (CD22) antibody
CD22 适用: 人, Non-Human Primate FACS, WB, IP 宿主: 小鼠 Monoclonal MEM-01 unconjugated

anti-CD22 Molecule (CD22) antibody
CD22 适用: 人, 小鼠, 大鼠, 猴, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4C3 unconjugated

anti-CD22 Molecule (CD22) (AA 20-687) antibody
CD22 适用: 人 FACS, WB, ELISA, IF, IP 宿主: 兔 Monoclonal DM13 unconjugated

anti-CD22 Molecule (CD22) antibody (PE)
CD22 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-01 PE

anti-CD22 Molecule (CD22) antibody (FITC)
CD22 适用: 人 FACS 宿主: 小鼠 Monoclonal IS7 FITC

anti-CD22 Molecule (CD22) (AA 52-178) antibody
CD22 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-022R unconjugated Recombinant Antibody

anti-CD22 Molecule (CD22) (AA 708-847) antibody
CD22 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/50 - 1/200

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Tuscano, Kato, Pearson, Xiong, Newell, Ma, Gandara, ODonnell: "CD22 antigen is broadly expressed on lung cancer cells and is a target for antibody-based therapy." in: Cancer research, Vol. 72, Issue 21, pp. 5556-65, (2012) (PubMed).

Kawasaki, Rademacher, Paulson: "CD22 regulates adaptive and innate immune responses of B cells." in: Journal of innate immunity, Vol. 3, Issue 4, pp. 411-9, (2011) (PubMed).
抗原

CD22 (CD22 Molecule (CD22))

别名

CD22

背景

CD22 may be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins, one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules

分子量

95.3 kDa

基因ID

933

UniProt

P20273