MBP 标记 抗体
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北京 101111
Quick Overview for MBP 标记 抗体 (ABIN1724707)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- MBP Antibody
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纯化方法
- Ascitic fluid
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免疫原
- Recombinant fusion protein with Maltose binding protein tag.
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亚型
- IgG1
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anti-MBP Tag antibody
WB 宿主: 兔 Polyclonal RB55937 unconjugated
anti-MBP Tag antibodyWB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-MBP Tag antibodyWB 宿主: 小鼠 Monoclonal 8K2 unconjugated
anti-MBP Tag antibodyWB, ELISA 宿主: 小鼠 Monoclonal 5B10B11 unconjugated
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应用备注
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ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/100 - 1/1000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Ascitic fluid containing 0.03 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
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: "Construction of a tagging system for subcellular localization of proteins encoded by open reading frames." in: Journal of biomedical science, Vol. 8, Issue 2, pp. 170-5, (2001) (PubMed).
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: "Construction of a tagging system for subcellular localization of proteins encoded by open reading frames." in: Journal of biomedical science, Vol. 8, Issue 2, pp. 170-5, (2001) (PubMed).
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- MBP Tag
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物质类
- Tag
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背景
- Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.
抗原
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