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Calreticulin 抗体

This anti-Calreticulin antibody is a 小鼠 单克隆 antibody detecting Calreticulin in WB, IHC, ELISA 和 ICC. Suitable for 人 和 小鼠. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN1724687
发货至: 中国

Quick Overview for Calreticulin 抗体 (ABIN1724687)

抗原

See all Calreticulin (CALR) 抗体
Calreticulin (CALR)

适用

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人, 小鼠

宿主

  • 137
  • 24
  • 2
  • 1
小鼠

克隆类型

  • 126
  • 38
单克隆

标记

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This Calreticulin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

克隆位点

1G6A7
  • 原理

    Calreticulin Antibody

    序列

    EEEDVPGQAK DELC

    纯化方法

    Ascitic fluid

    免疫原

    Synthetic peptide corresponding to aa (EEEDVPGQAKDELC) of human Calreticulin, conjugated to KLH.

    亚型

    IgG2a
  • 应用备注

    ELISA: 1/10000

    ICC: 1/200 - 1/1000

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Ascitic fluid containing 0.03 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
  • Tan, Chen, Li, Mabuchi, Bouvier: "The calcium- and zinc-responsive regions of calreticulin reside strictly in the N-/C-domain." in: Biochimica et biophysica acta, Vol. 1760, Issue 5, pp. 745-53, (2006) (PubMed).

  • 抗原

    Calreticulin (CALR)

    别名

    Calreticulin

    背景

    Calreticulin, also known as RO, CRT, SSA, cC1qR, FLJ26680, CALR. Entrez Protein NP_004334. It is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

    分子量

    48 kDa

    基因ID

    811

    NCBI登录号

    NP_004334

    UniProt

    P27797

    途径

    Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response
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