CpxA (N-Term) 抗体

Background: CpxA (Conjugative Plasmid Expression) is the 458 amino acid protein, a bacterial inner membrane histidine protein kinase, responsible for sensing misfolded proteins in the periplasm. It is part of CpxA/CpxR two component signal transduction system, where CpxR is the cognate response regulator. In sensing bacterial envelope stress, the Cpx pathway regulates the production of a number of periplasmic folding and trafficking factors to maintain outer membrane integrity. It is also responsible for global control of virulence factor expression. Alternative name: Integral membrane sensor signal transduction histidine kinase.

Additional information: The strains were grown in LB broth until late stationary phase (absorbance at 600nm of ~1,5), To avoid interference from cross-reacting bands, aim to maximize protein separation with the region of 35 to 55 kDa, CpxA could be routinely detected from a 20 μL of sample that was derived from 1 mL of pelleted bacteria resuspended in 200 μL of 1x loading buffer, Re-use of diluted working strength antibody is not recommended