CpxA (N-Term) 抗体
适用: Yersinia pseudotuberculosis
WB
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- CpxA
- 抗原表位
- N-Term
- 适用
- Yersinia pseudotuberculosis
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Western Blotting (WB)
- 交叉反应 （详细）
- Not reactive in: no confirmed exceptions from predicted reactivity are currently known
- 预测反应
- Yersinia pestis, Yersinia enterocolica and related species
- 纯化方法
- serum
- 免疫原
- Recombinant CpxA (residues 26 to 168) over-expressed as a N-terminal fusion with His(x6)-tag, which was removed prior to immunization. The antigen originates from enteropathogenic Yersinia pseudotuberculosis YPIII, B1JQV1, NCBI annotated locus tag YPK_4133.
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- 应用备注
- 1 : 2000 with standard ECL (WB)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 50 μL of sterile water.
- 注意事项
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Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. - 储存条件
- -20 °C
- 储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- 抗原
- CpxA
- 背景
- CpxA (Conjugative Plasmid Expression) is the 458 amino acid protein, a bacterial inner membrane histidine protein kinase, responsible for sensing misfolded proteins in the periplasm. It is part of CpxA/CpxR two component signal transduction system, where CpxR is the cognate response regulator. In sensing bacterial envelope stress, the Cpx pathway regulates the production of a number of periplasmic folding and trafficking factors to maintain outer membrane integrity. It is also responsible for global control of virulence factor expression. Alternative name: Integral membrane sensor signal transduction histidine kinase.
- 分子量
- expected: 51.8 kDa, appearent: 52-53 kDa
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