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Actin 抗体

This anti-Actin antibody is a 兔 多克隆 antibody detecting Actin in WB 和 IF. Suitable for Barley, Arabidopsis thaliana, Soybean 和 Zea mays. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN1720863
发货至: 中国

Quick Overview for Actin 抗体 (ABIN1720863)

抗原

See all Actin 抗体
Actin

适用

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Barley, Arabidopsis thaliana, Soybean, Zea mays

宿主

  • 87
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克隆类型

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多克隆

标记

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This Actin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF)
  • 特异性

    TAIR: At2g37620, At2g18780, At2g53750, At2g59370, At2g42100, At5g09810, At2g49240, At2g12110, At2g46520

    交叉反应 (详细)

    Not reactive in: Chlamydomonas reinhardtii (too high background for this species)

    预测反应

    Agropyron cristatum, Beta vulgaris, Betula luminifera, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Castanea sativa, Chorispora bungeana, Cucumis sativus, Cyanidioschyzon merolae strain 10D, Glycine max, Glycine soja, Halogeton glomeratus, Medicago truncatula, Malus domestica, Nicotiana tabacum, Oryza sativa, Pisum sativun, Solanum lysopersicum, Solanum tuberosum, Phaseolus vulgaris, Picea abies, Picea sitchensis, Prunus avium, Ricinus communis, Rubus plicatus, Theobroma cacao, Triticum aestivum, Vicia faba

    纯化方法

    serum

    免疫原

    ca. 100 amino acids of recombinant actin conserved more than 80 % in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47 AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293 AT1G49240 , actin-11 P53496 , AT3G12110 ,actin-12 P53497 AT3G46520
  • 应用备注

    1 : 2500 with standard ECL (WB), 1: 250 (IF)

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 50 μL of sterile water.

    注意事项

    Avoid repeated freeze-thaw cycles.
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

    储存条件

    -20 °C

    储存方法

    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Zheng, Fang, Pan, Sun, Deng, Grosser, Guo: "iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo." in: Journal of proteome research, Vol. 13, Issue 6, pp. 2998-3015, (2014) (PubMed).

  • 抗原

    Actin

    别名

    Actin (ACT)

    背景

    Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

    分子量

    expected: 41.6 kDa, appearent: 45 kDa

    UniProt

    P0CJ46, Q96292, P0CJ47, P53494, Q8RYC2, P53492, Q96293, P53496, P53497
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