Actin 抗体
Quick Overview for Actin 抗体 (ABIN1720863)
抗原
See all Actin 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- TAIR: At2g37620, At2g18780, At2g53750, At2g59370, At2g42100, At5g09810, At2g49240, At2g12110, At2g46520
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交叉反应 (详细)
- Not reactive in: Chlamydomonas reinhardtii (too high background for this species)
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预测反应
- Agropyron cristatum, Beta vulgaris, Betula luminifera, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Castanea sativa, Chorispora bungeana, Cucumis sativus, Cyanidioschyzon merolae strain 10D, Glycine max, Glycine soja, Halogeton glomeratus, Medicago truncatula, Malus domestica, Nicotiana tabacum, Oryza sativa, Pisum sativun, Solanum lysopersicum, Solanum tuberosum, Phaseolus vulgaris, Picea abies, Picea sitchensis, Prunus avium, Ricinus communis, Rubus plicatus, Theobroma cacao, Triticum aestivum, Vicia faba
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纯化方法
- serum
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免疫原
- ca. 100 amino acids of recombinant actin conserved more than 80 % in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47 AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293 AT1G49240 , actin-11 P53496 , AT3G12110 ,actin-12 P53497 AT3G46520
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应用备注
- 1 : 2500 with standard ECL (WB), 1: 250 (IF)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water.
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注意事项
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Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. -
储存条件
- -20 °C
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储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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: "iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo." in: Journal of proteome research, Vol. 13, Issue 6, pp. 2998-3015, (2014) (PubMed).
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: "iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo." in: Journal of proteome research, Vol. 13, Issue 6, pp. 2998-3015, (2014) (PubMed).
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- Actin
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别名
- Actin (ACT)
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背景
- Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
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分子量
- expected: 41.6 kDa, appearent: 45 kDa
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UniProt
- P0CJ46, Q96292, P0CJ47, P53494, Q8RYC2, P53492, Q96293, P53496, P53497
抗原
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