14-3-3 GRF 抗体
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北京 101111
Quick Overview for 14-3-3 GRF 抗体 (ABIN1720824)
抗原
适用
宿主
克隆类型
应用范围
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原理
- Anti-14-3-3 GRF | General regulatory element
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特异性
- TAIR: At4g09000, At4g78300, At4g38480, At4g35160, At4g16050, At4g10450, At4g02520, At4g65430, At4g42590, At4g22300, At4g34760
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交叉反应 （详细）
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Aesculus hippocastanum, Arabidopsis halleri, Arabidopsis thaliana, Lilium longiflorum, Mesembryanthemum crystallinum, Neochloris oloabundans (algae), Oryza sativa, Picrorhiza kurroa, Phaseolus vulgaris, Panax notoginseng, Zea mays, Vicia faba
Not reactive in: This antibody does not bind to 14-3-3-like protein D of Nicotiana tabacum. -
预测反应
- Brassica sp.,Chlamydomonas reinhardtii,Glycine max,Gossypium hirsutum,Nicotiana tabacum,Physcomitrium patens,Pisum sativum,Solanum tuberosum,Triticum aestivum
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纯化方法
- Serum
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免疫原
- Conserved surface-exposed peptide conjugated to KLH.This sequence is repeated in all 12 ""canonical"" members of the Arabidopsis thaliana GRF/14-3-3 family. GRF1 GF14 chi UniProt: F4JJ94
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应用备注
- 1: 2000 (WB)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- 14-3-3 GRF
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背景
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Background: GRF (General regulatory factor 2) or 14.3.3s are 30 kDa proteins involved in protein interactions with target proteins containing phosphorylated target sites. Functions of 14.3.3s include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system). A variety of apparently unrelated biological activities, including a role in development and signal transduction - brassinosteroid mediated signaling pathway.
Additional information: This antibody is recognizing recombiant GRF of Lilium longiflorum Lil1433_0 accession: AF191746, Lil1433_2, accession: EF397608 and recombinant GRF1,2,3,5 and 6 of Arabidopsis thaliana: GRF1 14-3-3 chi (At4g09000.1), GRF2 14-3-3 omega (At1g78300.1), GRF3 14-3-3 psi (At5g38480.1), GRF5 14-3-3 upsilon (At5g16050.1), GRF6 14-3-3 lambda (At5g10450.2), GRF8 14-3-3 kappa (At5g65430.1), GRF11 14-3-3 omicron (At1g34760.1).There is also very weak reaction to Physcomitrella patens Pp14-3-3 Pp1s 73_133V6 (closest homolog to AtGRF6) and Chlamydomonas reinhardtii Cr 14-3-3 Cre 12.g559250 (closest homolog to AtGRF6).
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分子量
- 20-28 kDa (depending upon an isoform)
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基因ID
- 818859, 831462
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UniProt
- F4JJ94, Q01525, F4KBI7, F4HWQ5, P42645, Q96300, F4KHY7, F4IP55, F4I1C1, Q9S9Z8, Q9C5W6
抗原
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