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pan Keratin 抗体

panKRT 适用: 人 IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal B311-1 unconjugated
产品编号 ABIN966802
发货至: 中国
  • 抗原 See all pan Keratin (panKRT) 抗体
    pan Keratin (panKRT)
    适用
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    小鼠
    克隆类型
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    单克隆
    标记
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    This pan Keratin antibody is un-conjugated
    应用范围
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    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    特异性
    This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond tocytokeratins 4,5,6,8,10,13 and 18.
    纯化方法
    Concentrated.
    免疫原
    Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.
    克隆位点
    B311-1
    亚型
    IgG1 kappa
    Top Product
    Discover our top product panKRT Primary Antibody
  • 应用备注
    IHC (FFPE): 1:50-1:100 (Requires high temp. antigen demaking, 10 mM Citrate pH 6.0 boiling tissue for 10-20 mins. followed by cooling at RT for 10-20 mins or protease digestion).
    Western blotting: 1:100-1:400
    Immunoprecipitation.
    Staining Procedure: This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC, with High temperature antigenic unmasking technique predigestion is recommended with this antibody when using paraffin-embedded tissue sections.
    说明

    Cellular Localization: Cytoplasmic.
    Recommended Positive Control: Skin, Colonic Carcinoma

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.35 mg/ml.
    缓冲液
    20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
  • 抗原
    pan Keratin (panKRT)
    别名
    Pan Cytokeratin, Pan CK-II (panKRT 产品)
    别名
    xk70a antibody, keratin antibody, keratin S homeolog antibody, LOC431321 antibody, krt.S antibody
    分子量
    45, 52.5 ,54, 56, 56.5, 58, & 59 kDa
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