pan Keratin 抗体
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- 抗原 See all pan Keratin (panKRT) 抗体
- pan Keratin (panKRT)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This pan Keratin antibody is un-conjugated
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond tocytokeratins 4,5,6,8,10,13 and 18.
- 纯化方法
- Concentrated.
- 免疫原
- Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.
- 克隆位点
- B311-1
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product panKRT Primary Antibody
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- 应用备注
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IHC (FFPE): 1:50-1:100 (Requires high temp. antigen demaking, 10 mM Citrate pH 6.0 boiling tissue for 10-20 mins. followed by cooling at RT for 10-20 mins or protease digestion).
Western blotting: 1:100-1:400
Immunoprecipitation.
Staining Procedure: This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC, with High temperature antigenic unmasking technique predigestion is recommended with this antibody when using paraffin-embedded tissue sections. - 说明
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Cellular Localization: Cytoplasmic.
Recommended Positive Control: Skin, Colonic Carcinoma - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.35 mg/ml.
- 缓冲液
- 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- pan Keratin (panKRT)
- 别名
- Pan Cytokeratin, Pan CK-II (panKRT 产品)
- 别名
- xk70a antibody, keratin antibody, keratin S homeolog antibody, LOC431321 antibody, krt.S antibody
- 分子量
- 45, 52.5 ,54, 56, 56.5, 58, & 59 kDa
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