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P4HB 抗体 (AA 52-63)

This anti-P4HB antibody is a 小鼠 单克隆 antibody detecting P4HB in WB, IHC, ELISA, IP, IF 和 ICC. Suitable for 人.
产品编号 ABIN1686617
发货至: 中国

Quick Overview for P4HB 抗体 (AA 52-63) (ABIN1686617)

抗原

See all P4HB 抗体
P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This P4HB antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

克隆位点

2E7-7
  • 抗原表位

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    AA 52-63

    特异性

    Detects ~48 kDa.

    交叉反应

    纯化方法

    Protein G Purified

    免疫原

    Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein

    亚型

    IgG2a
  • 应用备注

    • WB (1:200)
    • optimal dilutions for assays should be determined by the user.

    说明

    1 μg/ml of ABIN1686617 was sufficient for detection of EndoPDI in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    -20°C
  • 抗原

    P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

    别名

    PDI

    背景

    Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia (1).

    基因ID

    81567

    NCBI登录号

    NP_001139021

    UniProt

    Q8NBS9

    途径

    Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
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