HDAC4 抗体 (AA 600-650)
Quick Overview for HDAC4 抗体 (AA 600-650) (ABIN1607887)
抗原
See all HDAC4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 600-650
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Supplier Product No.
- 600-401-j20
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Supplier
- Rockland
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原理
- HDAC4 (internal) Antibody
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交叉反应 (详细)
- This antibody is specific towards HDAC4.
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产品特性
- Synonyms: rabbit anti-HDAC-4 antibody, HDAC4, HDAC 4, HD 4 antibody, histone deacetylase 4 antibody, Histone deacetylase-4, HD4 antibody
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纯化方法
- Anti-HDAC4 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: HDAC4 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding 600-650aa of human HDAC4.
Immunogen Type: Conjugated Peptide
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应用备注
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Immunohistochemistry Dilution: 1 mg/mL
Application Note: Anti-HDAC4 antibody has been tested in Western Blot and is useful for ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~140 kDa corresponding to the appropriate cell lysate or extract.
Western Blot Dilution: 1 mg/mL
ELISA Dilution: 1:20,000 - 1:60,000
IF Microscopy Dilution: 1:100-1:500
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 6 months
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- HDAC4 (Histone Deacetylase 4 (HDAC4))
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别名
- HDAC4
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背景
- Background: HDAC4 is a 140 kD class II histone deacetylase similar to the yeast HDA1 protein. This nuclear protein operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues. HDAC activity has been associated with transcriptional repression and nucleosomal condensation. HDAC4 uses active nuclear export to shuttle between the nucleus and the cytoplasm. HDAC4 is modified by phosphorylation by CaM kinase I or IV. HDAC4 has been reported to interact with 14-3-3 in the cytoplasm and MEF2A, HDAC3, NcoR and SMRT in the nucleus. Anti-HDAC4 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Cell Cycle and Replication, Chromatin Research, Epigenetics, and Histone Deacetylases research.
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基因ID
- 9759
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NCBI登录号
- NP_006028
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UniProt
- P56524
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process
抗原
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