ARHGAP22 抗体 (pSer397)
Quick Overview for ARHGAP22 抗体 (pSer397) (ABIN1607879)
抗原
See all ARHGAP22 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer397
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Supplier Product No.
- 600-401-j12
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Supplier
- Rockland
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原理
- Arhgap22 phospho S397 Antibody
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交叉反应 （详细）
- This antibody is specific for phosphorylated ARHGAP22 at Serine 397.
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产品特性
- Synonyms: rabbit anti-ARHGAP pS397 antibody, ARHGAP 22, ARHGAP-22, Rho-type GTPase-activating protein 22, RHOGAP2, Rho GTPase activating protein 22, rho GTPase-activating protein 22
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纯化方法
- Anti-ARHGAP22 pS397 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide corresponding to the region surrounding mouse pS397 region of ARHGAP22.
Immunogen Type: Conjugated Peptide
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anti-rho GTPase Activating Protein 22 (ARHGAP22) (C-Term) antibody
ARHGAP22 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-rho GTPase Activating Protein 22 (ARHGAP22) (AA 510-650) antibodyARHGAP22 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-rho GTPase Activating Protein 22 (ARHGAP22) (AA 604-633), (C-Term) antibodyARHGAP22 适用: 人 WB 宿主: 兔 Polyclonal RB32754 unconjugated
anti-rho GTPase Activating Protein 22 (ARHGAP22) (C-Term) antibodyARHGAP22 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:100-1:500
Application Note: Anti-ARHGAP22 pS397 antibody is tested for Western Blot and Immunostaining and useful for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77.8 kDa corresponding to the appropriate cell lysate or extract.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1:20,000 - 1:60,000
IF Microscopy Dilution: 1:100-1:500
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.07 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol with 1 mg/mL Bovine Serum Albumin (BSA)
Preservative: None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 6 months
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- ARHGAP22 (rho GTPase Activating Protein 22 (ARHGAP22))
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别名
- Arhgap22
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背景
- Background: ARHGAP22 is a Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. It acts as a GTPase activator for RAC1 by converting it to an inactive GDP-bound state and also inhibits RAC1-dependent lamellipodia formation. It may also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter. Anti-ARHGAP22 [p Ser397] antibody is ideal for researchers interested in Diabetes Research, Lipid and Metabolism research.
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基因ID
- 239027
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NCBI登录号
- NP_722495
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UniProt
- Q8BL80
抗原
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