Thymic Stromal Lymphopoietin 抗体 (Biotin)
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Quick Overview for Thymic Stromal Lymphopoietin 抗体 (Biotin) (ABIN1607864)
抗原
See all Thymic Stromal Lymphopoietin (TSLP) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 209-406-h48
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Supplier
- Rockland
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原理
- TSLP Antibody Biotin Conjugated
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交叉反应 (详细)
- This antibody is specific for human TSLP protein.
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产品特性
- Synonyms: rabbit anti-TSLP biotin conjugated antibody, rabbit anti-Thymic Stromal Lymphopoietin biotin conjugated antibody
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纯化方法
- Anti-TSLP is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Anti-TSLP antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human TSLP protein from E. coli.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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Labeling Ratio
- 10-20
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应用备注
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Application Note: TSLP purified antibody has been tested in western blotting and suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kDa in size corresponding to the human TSLP protein by western blotting in appropriate cell lysate or extract.
Western Blot Dilution: 1:1000-1:2000
ELISA Dilution: 1:20,000 - 1:50,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Thymic Stromal Lymphopoietin (TSLP)
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别名
- TSLP
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背景
- Background: Thymic Stromal Lymphopoietin (TSLP) is a hematopoietic cytokine. TSLP is mainly expressed in the heart, liver and prostate. TSLP sends signals through a heterodimeric receptor complex, containing the TSLP-R and the IL-7Rα chain to induce the release of T cell-attracting chemokines from monocytes. It can induce allergic inflammation by directly activating mast cells. It also promotes T helper type 2 (TH2) cell responses associated with immunity, including atopic dermatitis and asthma, and can promote TH2 cytokine-associated inflammation by directly promoting the effector functions of CD4+ TH2 cells, basophils and other granulocyte populations while simultaneously limiting the expression of dendritic cells derived proinflammatory cytokines and promoting regulatory T cell responses in peripheral tissues. Recombinant human TSLP is a non-glycosylated protein with a molecular weight of 15 kDa. Anti-TSLP antibody is ideal for researchers interested in cytokines and stem cell research.
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基因ID
- 85480
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NCBI登录号
- NP_149024
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UniProt
- Q969D9
抗原
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