RNASE1 抗体 (Biotin)
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Quick Overview for RNASE1 抗体 (Biotin) (ABIN1607821)
抗原
See all RNASE1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4688-0100
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Supplier
- Rockland
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原理
- Ribonuclease A Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine Pancreas].
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产品特性
- Synonyms: rabbit anti-Ribonuclease A Antibody biotin Conjugation, biotin Conjugated rabbit anti-Ribonuclease A Antibody, Pancreatic ribonuclease antibody, Rib 1 antibody, Rib1 antibody, Ribonuclease a family 1 antibody, Ribonuclease pancreatic antibody, Ribonuclease RNase A family 1 antibody, Ribonuclease RNase A family 1 pancreatic antibody
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纯化方法
- Anti-Ribonuclease A antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Ribonuclease A [Bovine Pancreas]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 26-152) antibody
RNASE1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (Center) antibodyRNASE1 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 29-156) antibodyRNASE1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 31-150) antibodyRNASE1 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) antibodyRNASE1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 14-42), (N-Term) antibodyRNASE1 适用: 人 WB 宿主: 兔 Polyclonal RB42410 unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 26-149) antibodyRNASE1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (AA 29-156) antibodyRNASE1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) antibodyRNASE1 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1) (C-Term) antibodyRNASE1 适用: 人, Cow, 大鼠, 山羊, 绵羊 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Ribonuclease A Biotin Conjugated antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Ribonuclease A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:13,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,500
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:150,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))
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别名
- RNASE1
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背景
- Background: Anti-Ribonuclease A recognizes ribonuclease, an endoribonuclease that catalyzes the cleavage of the phosphodiester bond between 5'-ribose of a nucleotide and the phosphate group of a 3'-ribose of a pyrimidine nucleotide. Able to survive acidic and near boiling conditions, RNase A is highly stable. RNase A and its homologs have been demonstrated to have cytotoxic and cytostatic effects.
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基因ID
- 282340
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UniProt
- P61823
抗原
-
