XDH 抗体 (Biotin)
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- 抗原 See all XDH 抗体
- XDH (Xanthine Dehydrogenase (XDH))
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适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This XDH antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)
- Supplier Product No.
- 200-4683-0100
- Supplier
- Rockland
- 原理
- Xanthine Oxidase Antibody Biotin Conjugated
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk].
- 产品特性
- Synonyms: rabbit anti-Xanthine Oxidase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Xanthine Oxidase Antibody, Xanthine dehydrogenase antibody, Xanthine dehydrogenase/oxidase antibody, Xanthine oxidase antibody, Xanthine oxidoreductase antibody, xdh antibody, xdha antibody, XO antibody, xor antibody
- 纯化方法
- Anti-Xanthine Oxidase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Xanthine Oxidase [Bovine Buttermilk]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product XDH Primary Antibody
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- 应用备注
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Application Note: Anti-Xanthine Oxidase Biotin conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Xanthine Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:250
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:500 - 1:2,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- XDH (Xanthine Dehydrogenase (XDH))
- 别名
- XDH (XDH 产品)
- 背景
- Background: Anti-Xanthine Oxidase recognizes xanthine oxidase, an oxidoreductase. Xanthine oxidase catalyzes the oxidation of hypoxanthine to xanthine and xanthine to uric acid. Both of these reactions also generate the reactive oxygen species hydrogen peroxide. In addition, xanthine oxidase is involved in the catabolism of purines and metabolism of caffeine. Clinically, xanthine oxidase has been linked to gout, a condition in which uric acid accumulates in the kidneys and joints. Anti-Xanthine Oxidase antibody is ideal for investigators interested in Metabolism and Signal Transduction research.
- 基因ID
- 280960
- UniProt
- P80457
- 途径
- Positive Regulation of Endopeptidase Activity
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