CES3 抗体 (Biotin)
Quick Overview for CES3 抗体 (Biotin) (ABIN1607803)
抗原
See all CES3 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4669-0100
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Supplier
- Rockland
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原理
- Esterase Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver].
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产品特性
- Synonyms: rabbit anti-Esterase Antibody biotin Conjugation, biotin conjugated rabbit anti-Esterase Antibody, Liver carboxylesterase, Proline-beta-naphthylamidase, Retinyl ester hydrolase
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纯化方法
- Anti-Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Esterase [Porcine Liver]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Carboxylesterase 3 (CES3) (AA 65-233) antibody
CES3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 3 (CES3) antibodyCES3 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 3 (CES3) (AA 1-568) antibodyCES3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Carboxylesterase 3 (CES3) (AA 269-568) antibodyCES3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 3 (CES3) (C-Term) antibodyCES3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Esterase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Esterase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: User Optimized
ELISA Dilution: 1:2,000 - 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CES3 (Carboxylesterase 3 (CES3))
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别名
- CES3
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背景
- Background: Anti-Esterase antibody is involved in the detoxification of xenobiotics, as well as in the activation of ester and amide prodrugs. The antibody is inactive on long-chain triacylglycerols and diacylglycerol, but active towards triacylglycerides composed with short-chain fatty acids from C2 to C6 and monoacylglycerols containing fatty acids from C2 to C12. Esterase hydrolyzes aromate and alkyl esters and vitamin A acetate, the rate of hydrolysis varies due to the hydrolyzing substances. Anti-Esterase antibody member of the type-B carboxylesterase/lipase family.
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基因ID
- 100626873, 343791015
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UniProt
- Q29550
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途径
- Monocarboxylic Acid Catabolic Process
抗原
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