DLD 抗体 (Biotin)
Quick Overview for DLD 抗体 (Biotin) (ABIN1607793)
抗原
See all DLD 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4660-0100
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Supplier
- Rockland
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原理
- Lipoamide Dehydrogenase Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart].
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产品特性
- Synonyms: rabbit anti-Lipoamide Dehydrogenase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Lipoamide Dehydrogenase Antibody, Dihydrolipoyl dehydrogenase mitochondrial, Dihydrolipoamide dehydrogenase, Lipoamide Dehydrogenase
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纯化方法
- Anti-Lipoamide dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Lipoamide Dehydrogenase [Porcine Heart]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 45-271) antibody
DLD 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyDLD 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyDLD 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyKO Validated DLD 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 1-509) antibodyDLD 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3C1 unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 36-300) antibodyDLD 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 238-466) antibodyDLD 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyDLD 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IF, FACS 宿主: 小鼠 Monoclonal 5G7 unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (C-Term) antibodyDLD 适用: 人, 小鼠, 大鼠 WB, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 280-487) antibodyDLD 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Lipoamide dehydrogenase Biotin Conjugated antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Lipoamide Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- DLD (Dihydrolipoamide Dehydrogenase (DLD))
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别名
- DLD LAD
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背景
- Background: Anti-Lipoamide dehydrogenase recognizes the protein lipoamide dehydrogenase. Lipoamide dehydrogenase catalyzes the conversion of lipoamide to dihydrolipoamide with the concomitant oxidation of NADH to NAD+. Lipoamide dehydrogenase is known to play a role in alpha-ketoacid dehydrogenase complexes as well glycine cleavage system. Defects in lipoamide dehydrogenase result in congenital infantile acidosis and maple syrup urine disease. Anti-Lipoamide dehydrogenase is suitable for investigators interested in metabolism, neurology, and cancer.
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基因ID
- 397129, 118675
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UniProt
- P09623
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途径
- Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis
抗原
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