ADH1 抗体 (Biotin)
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北京 101111
Quick Overview for ADH1 抗体 (Biotin) (ABIN1607773)
抗原
See all ADH1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4643-0100
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Supplier
- Rockland
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原理
- Alcohol Dehydrogenase Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast].
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产品特性
- Synonyms: rabbit anti-Alcohol Dehydrogenase Antibody biotin Conjugation, biotin conjugated rabbit ant-Alcohol Dehydrogenase Antibody, Alcohol dehydrogenase 1, Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, ADH, ADC
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纯化方法
- Anti-Alcohol Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Alcohol Dehydrogenase [Yeast]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) (AA 169-370) antibody
ADH1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) (AA 25-228) antibodyADH1 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) antibody (HRP)ADH1 适用: Saccharomyces cerevisiae WB, IHC, IP, ELISA 宿主: 兔 Polyclonal HRP
anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) antibodyADH1 适用: Saccharomyces cerevisiae WB, IP, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) antibodyADH1 适用: Saccharomyces cerevisiae WB, IP, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) antibodyADH1 适用: Saccharomyces cerevisiae WB, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Alcohol Dehydrogenase Biotin Conjugated Antibody has been tested in ELISA and Western blot. This product is assayed against 1.0 μg of Alcohol Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:2,000 - 1:15,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))
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别名
- ADH1
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背景
- Background: Alcohol Dehydrogenase is part of the zinc-containing alcohol dehydrogenase family. Anti-Alcohol Dehydrogenase catalyzes primary branched alcohols to convert to their aldehyde forms and shows some reactivity with secondary alcohols. Localized in the cytoplasm, the Anti-Alcohol Dehydrogenase antibody is ideal for researchers interested in Metabolism, Signal Transduction, and Cancer research.
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基因ID
- 2538902, 12643994
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UniProt
- P00330
抗原
-
