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- 抗原 See all Acid Phosphatase (ACP) 抗体
- Acid Phosphatase (ACP)
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适用
- Potato
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Acid Phosphatase antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应 （详细）
- Cross reactivity against Acid Phosphatase from other sources is unknown.
- 纯化方法
- Acid Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Acid Phosphatase [Potato]
Immunogen Type: Native Protein - 亚型
- IgG
- Top Product
- Discover our top product ACP Primary Antibody
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anti-Acid Phosphatase (ACP) antibody (HRP)
ACP 适用: Potato WB, ELISA 宿主: 兔 Polyclonal HRP
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- 应用备注
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Anti-Acid Phosphatase Biotin Conjugated Antibody has been assayed against 1.0 µg of Acid Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:8.000 to 1:32.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:4.000 - 1:20.000
Western Blot Dilution: 1:500 - 1:2.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 10 mg/mL
- 缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- Acid Phosphatase (ACP)
- 别名
- Acid Phosphatase (ACP 产品)
- 别名
- Acph antibody, ACP antibody, Acid phosphatase antibody, acid phosphatase antibody, cerebral palsy, ataxic 1 antibody, Dana\Acph antibody, phoC antibody, CPAT1 antibody
- 背景
- Acid Phosphatase is an enzyme which facilitates hydrolysis of alcohol groups on phosphate esters. Anti-Acid Phosphatase antibodies are ideal for investigators interested in Cancer, Enyzme, Cell Biology, and Cell Cycle Research. Synonyms: Acid phosphatase 1 soluble antibody, Acid phosphatase of erythrocyte antibody, Adipocyte acid phosphatase antibody, Cytoplasmic phosphotyrosyl protein phosphatase antibody, HAAP antibody
- UniProt
- Q6J5M8
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