Ovalbumin 抗体 (Biotin)
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北京 101111
Quick Overview for Ovalbumin 抗体 (Biotin) (ABIN1607759)
抗原
See all Ovalbumin (OVA) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4633-0100
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Supplier
- Rockland
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原理
- Ovalbumin Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].
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产品特性
- Synonyms: rabbit anti-Ovalbumin Antibody biotin Conjugation, biotin conjugated rabbit anti-Ovalbumin antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2, Hen Egg White
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纯化方法
- Anti-Ovalbumin Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Ovalbumin [Hen Egg White]
Immunogen Type: Native Protein
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亚型
- IgG
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Labeling Ratio
- 10-20
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anti-Ovalbumin (OVA) (AA 1-350) antibody
OVA 适用: 人, 小鼠, 大鼠 WB, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ovalbumin (OVA) antibody (HRP)OVA 适用: 小鸡 WB, ELISA, IP, IHC 宿主: 兔 Polyclonal HRP
anti-Ovalbumin (OVA) antibodyOVA 适用: Various Species WB 宿主: 小鼠 Monoclonal 3# unconjugated
anti-Ovalbumin (OVA) antibodyOVA 适用: Avian WB, ELISA 宿主: 小鼠 Monoclonal 3G2E1D9-3D5F4A8-3G1E1D9 unconjugated
anti-Ovalbumin (OVA) antibodyOVA 适用: 小鸡 WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Ovalbumin Antibody Biotin Conjugated is tested by ELISA, dot blot, and western blotting. In western blots anti-ovalbumin detects a single band of the expected apparent molecular weight. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:500 - 1:2,500
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Ovalbumin (OVA)
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别名
- SERPINB14
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背景
- Background: Ovalbumin Antibody Biotin Conjugated is specific for ovalbumin. Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65 % of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily. The function of ovalbumin is unknown, although it is presumed to be a storage protein or carrier protein.
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基因ID
- 396058
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UniProt
- P01012
抗原
-
