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beta-2 Microglobulin 抗体 (Biotin)

B2M 适用: 人 WB, ELISA, IHC, DB 宿主: 兔 Polyclonal Biotin
Rockland
产品编号 ABIN1607723
Supplier Product No.: 200-406-141-0100
发货至: 中国
  • 抗原 See all beta-2 Microglobulin (B2M) 抗体
    beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
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    标记
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    This beta-2 Microglobulin antibody is conjugated to Biotin
    应用范围
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)
    Supplier Product No.
    200-406-141-0100
    Supplier
    Rockland
    原理
    Beta-2-Microglobulin Antibody Biotin Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b2-Microglobulin [Human Urine].
    产品特性
    Synonyms: rabbit anti-Beta-2-Microglobulin Antibody biotin Conjugation, biotin conjugated Beta-2-Microglobulin Antibody, B2M antibody, Beta 2 microglobulin precursor antibody, Beta chain of mhc class 1 proteins antibody, Hdcma22p antibody
    纯化方法
    Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    免疫原

    Immunogen: Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.

    Immunogen Type: Native Protein

    亚型
    IgG
    Top Product
    Discover our top product B2M Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:500 - 1:3,000

    Application Note: Anti-beta-2-Microglobulin Biotin antibody has been tested by dot blot and is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

    Western Blot Dilution: 1:1,000 - 1:5,000

    ELISA Dilution: 1:2,000 - 1:10,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Keefe, Hwang, Huan, Mendelson, Yao, Courchesne, Saleh, Madhur, Levy: "Evidence for a Causal Role of the SH2B3-β2M Axis in Blood Pressure Regulation." in: Hypertension (Dallas, Tex. : 1979), Vol. 73, Issue 2, pp. 497-503, (2019) (PubMed).

  • 抗原
    beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
    别名
    B2M (B2M 产品)
    背景
    Background: Anti-beta-2-Microglobulin Antibody detects beta-2-Microglobulin. Beta-2-microglobulin is a component of the class I major histocompatibility complex (MHC), which are present on all nucleated cells (excludes red blood cells). It is involved in the presentation of peptide antigens to the immune system. Beta-2-microglobulin associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules such as CD1 and Qa. Defects in B2M are the cause of hypercatabolic hypoproteinemia. Anti-beta-2-Microglobulin Antibody is ideal for investigators involved in Cell Signaling, Immunology and Cell Biology research.
    基因ID
    567
    NCBI登录号
    NP_004039
    UniProt
    P61769
    途径
    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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