CPB1 抗体 (Biotin)
Quick Overview for CPB1 抗体 (Biotin) (ABIN1607707)
抗原
See all CPB1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-406-049-0100
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Supplier
- Rockland
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原理
- Carboxypeptidase B Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas].
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产品特性
- Synonyms: rabbit anti-Carboxypeptidase B antibody biotin conjugation, biotin conjugated rabbit anti-Carboxypeptidase B Antibody, CarboxypeptidaseB1 antibody, CP B1 antibody, CPB 1 antibody, CPB antibody, CPB1 antibody, DKFZp779K1333 antibody, Pancreas specific protein antibody, Pancreatic carboxypeptidase B1 antibody
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纯化方法
- Anti-CARBOXYPEPTIDASE B (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Carboxypeptidase B [Porcine Pancreas]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 111-417) antibody
CPB1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 109-415) antibodyCPB1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 14-417) antibodyCPB1 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 4D5 unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 1-417) antibodyCPB1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 9-37), (N-Term) antibodyCPB1 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB31698 unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) antibodyCPB1 适用: 人, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 16-45), (N-Term) antibodyCPB1 适用: 人 WB, IHC (p), IF, FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 1-417) antibodyCPB1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (C-Term) antibodyCPB1 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase B1 (Tissue) (CPB1) (AA 120-415) antibodyCPB1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-CARBOXYPEPTIDASE B Biotin Antibody has been tested by Western Blot and is suitable to be assayed against 1.0 μg of Carboxypeptidase B in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))
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别名
- CPB1
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背景
- Background: A carboxypeptidase (EC number 3.4.16 - 3.4.18) is a protease enzyme that hydrolyzes (cleaves) a peptide bond at the carboxy-terminal (C-terminal) end of a protein or peptide. (Contrast with an aminopeptidase, which cleaves peptide bonds at the other end of the protein.) Humans, animals, and plants contain several types of carboxypeptidases that have diverse functions ranging from catabolism to protein maturation. Carboxypeptidase B is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid.
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基因ID
- 397341
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NCBI登录号
- NP_999334
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UniProt
- P09955
抗原
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