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beta-2 Microglobulin 抗体

The 兔 多克隆 anti-beta-2 Microglobulin antibody is suitable to detect beta-2 Microglobulin in samples from 人. It has been validated for WB, IHC, IF, ELISA 和 FM.
Rockland
产品编号 ABIN1607703
Supplier Product No.: 200-401-141-0100
发货至: 中国
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Quick Overview for beta-2 Microglobulin 抗体 (ABIN1607703)

抗原

See all beta-2 Microglobulin (B2M) 抗体
beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

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多克隆

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This beta-2 Microglobulin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Fluorescence Microscopy (FM)
  • Supplier Product No.

    200-401-141-0100

    Supplier

    Rockland

    原理

    Beta-2-Microglobulin Antibody

    交叉反应 (详细)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum, as well as purified and partially purified b2-Microglobulin (Human Urine).

    产品特性

    Synonyms: rabbit anti-Beta-2-Microglobulin Antibody, b2-Microglobulin, B2M antibody, B2MG_HUMAN antibody, Beta 2 microglobin antibody, Beta 2 microglobulin precursor antibody, Beta chain of mhc class 1 proteins antibody, Hdcma22p antibody, CDABP0092 antibody, IMD43 antibody

    纯化方法

    Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    免疫原

    Immunogen: Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with human beta-2-Microglobulin protein isolated from urine.

    Immunogen Type: Native Protein

    亚型

    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:500 - 1:2,000

    Application Note: Anti-beta-2-Microglobulin antibody has been tested in western blot, immunohistochemistry, and immunofluorescence. This product detects a single band of the expected apparent molecular weight (~13.7 kDa), and is suitable for use in ELISA. Researchers should determine optimal titers for applications that are not stated below.

    Western Blot Dilution: 1:200 - 1:500

    ELISA Dilution: 1:2,000 - 1:10,000

    IF Microscopy Dilution: 1:200

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • 抗原

    beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

    别名

    B2M

    背景

    Background: Anti-beta-2-Microglobulin Antibody detects beta-2-Microglobulin. Beta-2-microglobulin is a component of the class I major histocompatibility complex (MHC), which are present on all nucleated cells (excludes red blood cells). It is involved in the presentation of peptide antigens to the immune system. Beta-2-microglobulin associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules such as CD1 and Qa. Defects in B2M are the cause of hypercatabolic hypoproteinemia. Anti-beta-2-Microglobulin Antibody is ideal for investigators involved in Cell Signaling, Immunology and Cell Biology research.

    基因ID

    567

    NCBI登录号

    NP_004039

    UniProt

    P61769

    途径

    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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