PKM 抗体 (Biotin)
Quick Overview for PKM 抗体 (Biotin) (ABIN1607677)
抗原
See all PKM 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-1678-0100
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Supplier
- Rockland
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原理
- Anti-Pyruvate Kinase Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle].
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产品特性
- Synonyms: goat anti-Pyruvate Kinase Antibody, biotin Conjugated goat anti-Pyruvate Kinase Antibody, Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, PKM2
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纯化方法
- Anti-Pyruvate Kinase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Pyruvate Kinase [Rabbit Muscle]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Pyruvate Kinase M1/2 (PKM) (AA 2-531) antibody
PKM 适用: 人 ELISA, WB, IHC, IF, IP, FACS 宿主: 小鼠 Monoclonal 6C3C7 unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (AA 11-221) antibodyPKM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) antibodyPKM 适用: 人 ELISA, WB, IHC, IF, IP, FACS 宿主: 兔 Monoclonal 7B2 unconjugated Recombinant Antibody
anti-Pyruvate Kinase M1/2 (PKM) (AA 185-461) antibodyPKM 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (AA 11-221) antibodyPKM 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (Internal Region) antibodyPKM 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (AA 2-192) antibodyPKM 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (pSer37) antibodyPKM 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) antibodyPKM 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (pSer37) antibodyPKM 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Pyruvate Kinase Biotin Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody.
Western Blot Dilution: 1:200 - 1:1,000
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- PKM (Pyruvate Kinase M1/2 (PKM))
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别名
- PKM
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背景
- Background: Pyruvate Kinase is an enzyme that is key in the glycolytic process. Pyruvate Kinase's specific activity is to catalyze the transfer of a phosphate group from phosphoenolpyruvate to ADP, yielding one molecule of pyruvate and one molecule of ATP. A isoenzyme of Pyruvate Kinase, known as the M2, is a key area of study in tumorigenesis, as the M2 isoenzyme seems to elevate aerobic glycolysis in tumor cells, leading to tumor proliferation. Conflicting evidence as to the importance of this isoenzyme in tumor growth establishes this as a large area of exploration and study. Anti-Pyruvate Kinase Antibody is ideal for investigators in Cancer, Cell Biology, and Signal Transduction research.
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基因ID
- 100008676
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NCBI登录号
- NP_001182574
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UniProt
- P11974
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途径
- Warburg Effect
抗原
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