CA1 抗体 (Biotin)
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北京 101111
Quick Overview for CA1 抗体 (Biotin) (ABIN1607667)
抗原
See all CA1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-1654-0100
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Supplier
- Rockland
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原理
- Carbonic Anhydrase I Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes].
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产品特性
- Synonyms: goat anti-Carbonic Anhydrase I Antibody biotin Conjugation, biotin Conjugated goat anti-Carbonic Anhydrase I Antibody, Car1 antibody, Carbonate dehydratase I antibody, Carbonic anhydrase 1 antibody, Carbonic anhydrase B antibody, Carbonic dehydratase antibody, ECK0125 antibody, yadF antibody
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纯化方法
- Anti-CARBONIC ANHYDRASE I (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Carbonic Anhydrase I [Human Erythrocytes]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Carbonic Anhydrase I (CA1) (AA 1-261) antibody
CA1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal M1 unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 2-261) antibodyCA1 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 1-261) antibodyCA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F1 unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 1-261) antibodyCA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal M2 unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 1-261) antibodyCA1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 1-261) antibodyCA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase I (CA1) (Internal Region) antibodyCA1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase I (CA1) (N-Term) antibodyCA1 适用: 人, Cow, 豚鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 25-90) antibodyCA1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 9D6D7 unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 2-261) antibodyCA1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Carbonic anhydrase I Biotin Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Carbonic Anhydrase I in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Specific conditions should be optimized by researcher.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CA1 (Carbonic Anhydrase I (CA1))
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别名
- CA1
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背景
- Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. Carbonic Anhydrase 1 (CA1) is closely related to Carbonic Anhydrase 2 (CA2) and Carbonic Anhydrase 3 (CA3), and it is a cytosolic protein that is found at the highest level in erythrocytes. Anti-Carbonic anhydrase I antibody is ideal for investigators involved in serum protein components.
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基因ID
- 759
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NCBI登录号
- NP_001122301
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UniProt
- P00915
抗原
-
