GLN1 抗体 (Biotin)
Quick Overview for GLN1 抗体 (Biotin) (ABIN1607650)
抗原
See all GLN1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-106-238-0100
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Supplier
- Rockland
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原理
- Glutamine Synthetase Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial].
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产品特性
- Synonyms: goat anti-Glutamine Synthetase Antibody biotin Conjugation, biotin Conjugated goat anti-Glutamine Synthetase Antibody, Glutamine synthetase
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纯化方法
- Anti-Glutamine Synthetase (microbial) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Glutamine Synthetase [Microbial]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Glutamine Synthetase (GLN1) (AA 1-373) antibody
GLN1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) (AA 1-373) antibodyGLN1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) (AA 285-373) antibodyGLN1 适用: 人, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) (AA 50-250) antibodyGLN1 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-750M unconjugated
anti-Glutamine Synthetase (GLN1) (AA 1-373) antibodyGLN1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamine Synthetase (GLN1) antibodyGLN1 适用: 小鼠, 大鼠 WB, IHC 宿主: 豚鼠 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Glutamine Synthetase (microbial) antibody has been assayed against 1.0 μg of Glutamine Synthetase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,500
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- GLN1 (Glutamine Synthetase (GLN1))
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别名
- Glutamine Synthetase
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背景
- Background: Glutamine Synthetase is a key enzyme in the metabolism of nitrogen. Glutamine synthetase catalyzes an ATP-dependent condensation reaction between ammonia and glutamate to yield glutamine. Glutamine is a key builder of proteins as well as a vehicle to deliver nitrogen atoms to enzymes that build molecules dependent on nitrogen. Glutamine Synthetase from a microbial source is composed of twelve subunits that each house an active site. During the reaction of glutamine synthetase, the active sites bind ammonia and glutamate, as well as an ATP molecule to power the reaction. Negative feedback regulation is provided by the active sites ability to weakly bind other molecules and once their concentrations rise too high, the enzyme shuts off. Glutamine Synthetase has applications in neuroscience due to location in astrocytes within the brain and fluctuations in glutamine synthetase can detrimentally effect the astrocytes. Anti-Glutamine Synthetase (Microbial) Antibody is ideal for investigators in Molecular Biology, Neuroscience, and Enzymology.
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基因ID
- 3345165
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UniProt
- Q79VE3
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途径
- Positive Regulation of Peptide Hormone Secretion
抗原
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