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- 抗原 See all HCP products
- HCP (Host Cell Proteins (HCP))
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适用
- E. coli
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HCP antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 原理
- Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli.
- 特异性
- E.coli HCP Antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins. This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. The anti-sera reactivity is directed against E.coli Host Cell Proteins.
- 交叉反应 （详细）
- Cross reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
- 产品特性
- Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate. Synonyms: Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, ECP antibody, Anti-ECP
- 纯化方法
- This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. Reactivity is directed against E.coli Host Cell Proteins.
- 过滤
- Sterile filtered
- 免疫原
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Anti-Host Cell Proteins Antibody was prepared combining antibodies that have been separatly developed by repeated immunizations with a mixture of high molecular weight (HMW) and a mixture of low molecular weight (LMW) proteins from E.Coli.
Immunogen Type: Native Protein
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anti-Host Cell Proteins (HCP) antibody
HCP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Host Cell Proteins (HCP) antibodyHCP 适用: Chinese Hamster WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Host Cell Proteins (HCP) antibodyHCP 适用: E. coli WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot Dilution: 1:500 - 1:1000
ELISA : 1:1000-1:2000 - 说明
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Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and is suitable for western blotting and ELISA.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 77.5 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store Host Cell Proteins Antibody at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- HCP (Host Cell Proteins (HCP))
- 别名
- Host Cell Proteins (HCP 产品)
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