KIF11 抗体
Quick Overview for KIF11 抗体 (ABIN1607602)
抗原
See all KIF11 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-401-j04
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Supplier
- Rockland
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原理
- Eg5 Antibody
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交叉反应 （详细）
- Anti-Eg5 is directed against the human Eg5 protein.
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产品特性
- Synonyms: rabbit anti-Eg5 antibody, Kinesin-like protein KIF11, Kinesin-5, KIF11, Kinesin-related motor protein Eg5, Thyroid receptor-interacting protein 5
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纯化方法
- The product was prepared from monospecific antiserum by delipidation and defibrination.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Eg5 was prepared from whole rabbit serum produced by repeated immunizations with a truncated Eg5 construct expressed in E. coli corresponding to human Eg5 protein.
Immunogen Type: Recombinant Protein
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anti-Kinesin Family Member 11 (KIF11) antibody
KIF11 适用: 人 WB, IP, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Kinesin Family Member 11 (KIF11) (AA 103-130), (N-Term) antibodyKIF11 适用: 人, Hamster WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB22568 unconjugated
anti-Kinesin Family Member 11 (KIF11) (AA 920-1038) antibodyKIF11 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Kinesin Family Member 11 (KIF11) (Ser711) antibodyKIF11 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Kinesin Family Member 11 (KIF11) (C-Term) antibodyKIF11 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Kinesin Family Member 11 (KIF11) (AA 797-1056) antibodyKIF11 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Kinesin Family Member 11 (KIF11) (C-Term), (pSer926) antibodyKIF11 适用: 人 WB, IF, IHC, IC 宿主: 兔 Polyclonal unconjugated
anti-Kinesin Family Member 11 (KIF11) (AA 892-941) antibodyKIF11 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Kinesin Family Member 11 (KIF11) (AA 109-139), (N-Term) antibodyKIF11 适用: 人 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Kinesin Family Member 11 (KIF11) antibodyKIF11 适用: 人 WB, ELISA 宿主: 兔 Monoclonal 1G11 unconjugated Recombinant Antibody
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Eg5 antibody has been tested by western blot. For western blots expect a band of approximately 72 kDa in size corresponding to truncated kinesin-1 protein. Specific conditions for reactivity should be optimized by the end user. This antibody is suitable for use in ELISA.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 46 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- KIF11 (Kinesin Family Member 11 (KIF11))
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别名
- KIF11
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背景
- Background: Eg5 (also known as kinesin-5 and KIF11) is a homo-tetramer which cross-links anti-parallel microtubules in the mitotic spindle to maintain spindle bipolarity. Eg5, a member of the Kinesin-5 subclass of kinesins, is a plus-end-directed tetrameric kinesin-family protein that influences the assembly and organization of the mitotic spindle, a self-assembled and dynamic microtubule-based structure that orchestrates chromosome segregation in dividing cells. Eg5 action is essential: when it is depleted from the cytoplasm of meiotically-mature Xenopus laevis eggs, abnormal monopolar spindles form, preventing successful division. Eg5 is expressed in all cells during mitosis and in post-mitotic neurons during development. In developing neurons pharmacological inhibition and siRNA knockdown of Eg5 results in longer axons, more branches, fewer bouts of axon retraction and the inability of growth cones to turn on contact with repulsive substrates. In migratory neurons, inhibition of Eg5 causes neurons to migrate in a random pattern and form shorter leading processes. In adult neurons, Eg5 has a similar effect on inhibiting the rate of short microtubule transport so pharmacological inhibition of adult Eg5 (i.e Monastrol) may be a potential therapeutic tool for the augmentation of adult axon regeneration.
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基因ID
- 3799
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NCBI登录号
- NP_004512
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UniProt
- P52732
抗原
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