Myosin VI 抗体
Quick Overview for Myosin VI 抗体 (ABIN1607600)
抗原
See all Myosin VI (MYO6) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-401-j03
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Supplier
- Rockland
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原理
- Myosin-6 Antibody
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交叉反应 （详细）
- Anti-Myosin-6 is directed against the myosin-6 protein.
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产品特性
- Synonyms: rabbit anti-Myosin-6 Antibody, MyosinVI, Unconventional myosin 6
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纯化方法
- The product was prepared from monospecific antiserum by delipidation and defibrination.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Myosin-6 was prepared from whole rabbit serum produced by repeated immunizations with full length myosin-6 construct expressed in SF9 insect cells corresponding to porcine myosyn-6 protein.
Immunogen Type: Recombinant Protein
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anti-Myosin VI (MYO6) (N-Term) antibody
MYO6 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Myosin VI (MYO6) (AA 1188-1285) antibodyMYO6 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E12 unconjugated
anti-Myosin VI (MYO6) (AA 1166-1195), (C-Term) antibodyMYO6 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB19127 unconjugated
anti-Myosin VI (MYO6) (AA 1173-1203), (C-Term) antibodyMYO6 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Myosin VI (MYO6) (N-Term) antibodyMYO6 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myosin VI (MYO6) (AA 1016-1285) antibodyMYO6 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Myosin-6 antibody has been tested by western blot. For western blots expect a band of approximately 150 kDa in size corresponding to full length and ~85 kDa and ~60 kDa for truncated myosin-6 protein. Specific conditions for reactivity should be optimized by the end user. This antibody is suitable for use in ELISA.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:10,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 25 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Myosin VI (MYO6)
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别名
- MYO6
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背景
- Background: Myosin VI is a myosin superfamily member with unique and intriguing features that allow it to fill a still-expanding number of cell biological roles. This actin-based motor produces force that acts towards the minus end of actin filaments, which is the opposite direction to all other characterized myosins. In mammalian cells, myosin VI is localized to endocytic vesicles, membrane ruffles, the cytosol and the Golgi complex. Its motor function is essential for several physiological functions of the cell, including normal rates of endocytosis, maintenance of Golgi morphology and protein secretion. Myosin VI regulates epithelial cell migration and plays a role in the maintenance of adhesive cellular contacts within epithelial cell layers. It is highly expressed in ovarian cancers and prostate cancers and its expression level, which is upregulated by DNA damage in a p53-dependent manner, correlates with the potential of the tumor to disseminate. More recently, myosin VI has been found involved in EGFR endocytosis through a clathrin dependent mechanism.
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基因ID
- 397085
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NCBI登录号
- NP_999186
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UniProt
- Q29122
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途径
- Sensory Perception of Sound, Dicarboxylic Acid Transport, Asymmetric Protein Localization
抗原
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