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- 抗原 See all ARFGAP3 抗体
- ARFGAP3 (ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ARFGAP3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM)
- 交叉反应 （详细）
- Cross reactivity with human, monkey, and orangutan for ArfGAP3. Cross reactivity with AfGAP3 from other sources have not been determined.
- 纯度
- Anti-ArfGAP3 atibody was prepared from monospecific antiserum by delipidation and defibrination. Further purification was used to remove the GST tag. The antibody detects ArfGAP3 in cell lysates.
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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This whole rabbit serum was prepared by repeated immunizations with a truncated recombinant sequence of ArfGAP3 fused to GST.
Immunogen Type: Recombinant Protein - Top Product
- Discover our top product ARFGAP3 Primary Antibody
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anti-ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3) (AA 217-516) antibody
ARFGAP3 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3) (C-Term) antibodyARFGAP3 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3) antibodyARFGAP3 适用: 人, 小鼠 WB 宿主: 山羊 Polyclonal unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3) (C-Term) antibodyVerified ARFGAP3 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3) (AA 377-426) antibodyARFGAP3 适用: 人, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3) (AA 91-140) antibodyARFGAP3 适用: 人, 豚鼠, 马, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ArfGAP3 has been tested for use in ELISA, Immunofluorescence, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57 kDa in size by western blotting in the appropriate cell lysate or extract.
ELISA Dilution: 1:5.000
IF Microscopy Dilution: 1:100
Western Blot Dilution: 1:1.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- ARFGAP3 (ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3))
- 别名
- ArfGAP3 (ARFGAP3 产品)
- 别名
- ARFGAP1 antibody, 0610009H19Rik antibody, 1810004P07Rik antibody, 1810035F16Rik antibody, 9130416J18Rik antibody, Arfgap1 antibody, arfgap1 antibody, xgap antibody, zgc:85765 antibody, arfgap3 antibody, ADP ribosylation factor GTPase activating protein 3 antibody, ADP-ribosylation factor GTPase activating protein 3 antibody, ADP ribosylation factor GTPase activating protein 3 S homeolog antibody, ARFGAP3 antibody, Arfgap3 antibody, arfgap3 antibody, arfgap3.S antibody
- 背景
- The ArfGAPs are a family of proteins encoded by 31 genes in humans. The function of the ArfGAPs is to regulate the small G protein Arf, a regulator of membrane traffic and actin cytoskeleton. The ArfGAPs have been found to be key regulators of cellular behaviors that involve coordinated actin and membrane remodeling, including protein secretion and migration. Several ArfGAPs are associated with cancer cell invasion and metastasis, and the Arf pathway has been found to be affected in a number of genetic diseases. Although the importance of the ArfGAPs and the Arf pathway for cellular physiology is well recognized, the molecular basis for the function of these proteins has not been established. Reagents for the studies, especially antibodies specific for particular members of the ArfGAP family that can be used for immunoblotting and immunofluorescence, are not available. ArfGAP3 is one of three ArfGAPs thought to be specifically associated with the Golgi apparatus, however, localization has only been done for ectopically expressed recombinant protein and cellular function is still not established. Indeed, there is a growing controversy about the function of ArfGAP3 together with ArfGAP1 and ArfGAP2. Antibodies suitable for immunoprecipitation, immunoblotting and immunofluorescence would be of great value in addressing the controversy and for discovering the potential role of ArfGAP3 in secretion of proteins, such as growth factors, and trafficking of other proteins to the cell surface, such as growth factor receptors and cell adhesion molecules. Synonyms: ARFGAP1, ADP-ribosylation factor GTPase-activating protein 3, ARF GAP3, Arf-GAP
- 基因ID
- 26286
- NCBI登录号
- NP_001135765
- UniProt
- Q9NP61
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