KRIT1 抗体
Quick Overview for KRIT1 抗体 (ABIN1589846)
抗原
See all KRIT1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
原理
- CCM-1 antibody
-
特异性
- Recombinant human CCM1
-
产品特性
- Chromosomal location: 7q21.2
-
纯化方法
- Protein A purified
-
免疫原
- Recombinant human CCM1 (ABIN7539340)
-
亚型
- IgG
-
-
anti-KRIT1, Ankyrin Repeat Containing (KRIT1) (AA 631-736) antibody
KRIT1 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-KRIT1, Ankyrin Repeat Containing (KRIT1) (AA 471-585) antibodyKRIT1 适用: 人 ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-KRIT1, Ankyrin Repeat Containing (KRIT1) (AA 637-736) antibodyKRIT1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-KRIT1, Ankyrin Repeat Containing (KRIT1) (AA 637-736) antibodyKRIT1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2C7 unconjugated
anti-KRIT1, Ankyrin Repeat Containing (KRIT1) (AA 637-736) antibodyKRIT1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1D8 unconjugated
anti-KRIT1, Ankyrin Repeat Containing (KRIT1) (AA 1-736), (N-Term) antibodyKRIT1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Western Blot: Use 1-5 μg/mL
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
-
缓冲液
- 0.5X PBS, pH 7.2
-
注意事项
- Centrifuge vial prior to opening. Avoid repeated freeze-thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
-
有效期
- 24 months
-
-
- KRIT1 (KRIT1, Ankyrin Repeat Containing (KRIT1))
-
别名
- CCM-1
-
背景
- CCM-1, Cerebral cavernous malformations protein 1, KRIT1, KRIT1, ankyrin repeat containing, CAM,Cerebral cavernous malformations (CCM) are frequent vascular abnormalities caused by mutations in one of the CCM genes. CCM-1 (also known as KRIT1) stabilizes endothelial junctions and is essential for vascular morphogenesis in mouse embryos. However, cellular functions of CCM-1 during the early steps of the CCM pathogenesis remain unknown. It was shown that CCM-1 represents an antiangiogenic protein to keep the human endothelium quiescent. CCM-1 inhibits endothelial proliferation, apoptosis, migration, lumen formation, and sprouting angiogenesis in primary human endothelial cells. CCM-1 strongly induces DLL4-NOTCH signaling, which promotes AKT phosphorylation but reduces phosphorylation of the mitogen-activated protein kinase ERK. Consistently, blocking of NOTCH activity alleviates CCM-1 effects. ERK phosphorylation is increased in human CCM lesions. Transplantation of CCM-1-silenced human endothelial cells into SCID mice recapitulates hallmarks of the CCM pathology and serves as a unique CCM model system.
-
基因ID
- 889, 3
-
NCBI登录号
- NM_004912, NP_004903
-
UniProt
- O00522
-
途径
- Cell RedoxHomeostasis
抗原
-
