The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
The antiserum was produced against synthesized phosphopeptide derived from human ERK8 around the phosphorylation site of threonine 175 and tyrosine 177 (A-V-TP-E-YP-V-A).