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- 抗原
- Ty1 Tag
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 序列
- EVHTNQDPLD
- 特异性
- This Antibody recognizes both N-terminal and C-terminal Ty1 tagged fusion proteins.
- 纯化方法
- Immunoaffinity chromatography
- 免疫原
- Ty1-tag epitope peptide conjugated - KLH
- 亚型
- IgG
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anti-Ty1 Tag antibody
WB 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.2-2 µg/mLImmunoprecipitation (IP): 2-5 µg/mL
Other applications: user-optimized - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4, containing 0.02 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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- 抗原
- Ty1 Tag
- 物质类
- Tag
- 背景
- Well-characterized antibodies for epitope tags are common in the study of protein expression in various systems.Rabbit Anti- Ty1-tag Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide.
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