V5 Epitope Tag 抗体 (HRP)
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北京 101111
Quick Overview for V5 Epitope Tag 抗体 (HRP) (ABIN1573948)
抗原
适用
宿主
克隆类型
标记
应用范围
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序列
- GKPIPNPLLG LDST
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特异性
- Rabbit Anti-V5-tag [HRP] Polyclonal Antibody specifically recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
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纯化方法
- Immunoaffinity chromatography
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免疫原
- V5 epitope tag peptide GKPIPNPLLGLDST conjugated - KLH
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亚型
- IgG
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anti-V5 Epitope Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 8A7A8 unconjugated
anti-V5 Epitope Tag antibodyWB 宿主: 山羊 Polyclonal unconjugated
anti-V5 Epitope Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-V5 Epitope Tag antibodyWB, IP, IF, IC 宿主: 小鼠 Monoclonal unconjugated
anti-V5 Epitope Tag antibodyWB 宿主: 兔 Monoclonal 25GB7075 unconjugated Recombinant Antibody
anti-V5 Epitope Tag antibody适用: Paramyxovirus WB, ELISA, IP, IF, IHC (fro), RIA 宿主: 小鼠 Monoclonal SV5-P-K unconjugated Recombinant Antibody
anti-V5 Epitope Tag antibodyWB, ELISA, IHC 宿主: 小鼠 Monoclonal E10 unconjugated
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应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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: "An eEF1A1 truncation encoded by PTI-1 exerts its oncogenic effect inside the nucleus." in: Cancer cell international, Vol. 14, Issue 1, pp. 17, (2014) (PubMed).
: "Caveolin-1-LRP6 signaling module stimulates aerobic glycolysis in prostate cancer." in: Cancer research, Vol. 73, Issue 6, pp. 1900-11, (2013) (PubMed).
: "The C8ORF38 homologue Sicily is a cytosolic chaperone for a mitochondrial complex I subunit." in: The Journal of cell biology, Vol. 200, Issue 6, pp. 807-20, (2013) (PubMed).
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: "An eEF1A1 truncation encoded by PTI-1 exerts its oncogenic effect inside the nucleus." in: Cancer cell international, Vol. 14, Issue 1, pp. 17, (2014) (PubMed).
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- V5 Epitope Tag
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别名
- V5-Tag
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物质类
- Tag
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背景
- Rabbit Anti-V5-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Rabbit Anti-V5-tag [HRP] polyclonal antibody is suitable for detecting fusion proteins that contain V5-tags.
抗原
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