VSV-g Tag 抗体
(5 references)-
- 抗原 See all VSV-g Tag products
- VSV-g Tag (VSV-G Epitope Tag)
- 适用
- Vesicular Stomatitis Virus (VSV)
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This VSV-g Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 序列
- YTDIEMNRLG K
- 特异性
- This Antibody recognizes C-terminal, N-terminal, and internal VSV-G tagged fusion proteins.
- 纯化方法
- Immunoaffinity chromatography
- 免疫原
- VSV-G epitope tag peptide YTDIEMNRLGK conjugated - KLH
- 亚型
- IgG1
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anti-VSV-G Epitope Tag antibody
适用: Vesicular Stomatitis Virus (VSV) WB, IF, IP 宿主: 小鼠 Monoclonal 8D6 unconjugated
anti-VSV-G Epitope Tag antibody适用: Vesicular Stomatitis Virus (VSV) WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-VSV-G Epitope Tag antibodyWB, IF, IP 宿主: 小鼠 Monoclonal 1H7 unconjugated
anti-VSV-G Epitope Tag antibody适用: Vesicular Stomatitis Virus (VSV) ELISA, FM 宿主: 兔 Polyclonal unconjugated
anti-VSV-G Epitope Tag antibody适用: Vesicular Stomatitis Virus (VSV) WB, IF, IP, IC 宿主: 小鼠 Monoclonal unconjugated
anti-VSV-G Epitope Tag antibody (HRP)适用: Vesicular Stomatitis Virus (VSV) WB, ELISA 宿主: 兔 Polyclonal HRP
anti-VSV-G Epitope Tag (AA 501-511) antibody适用: Vesicular Stomatitis Virus (VSV) WB 宿主: 山羊 Polyclonal unconjugated
anti-VSV-G Epitope Tag antibody (Biotin)适用: Vesicular Stomatitis Virus (VSV) ELISA, FM 宿主: 兔 Polyclonal Biotin
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by severalfactors, including secondary antibody affinity, antigen concentration,sensitivity of detection method, temperature, and length of incubations,etc. The suitability of this antibody for applications other than thoselisted below has not been determined. The following concentration rangesare recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Immunoprecipitation (IP): 2-10 µg/mg of lysateOther applications: user-optimized - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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: "Mitogen-activated protein kinase-mediated licensing of interferon regulatory factor 3/7 reinforces the cell response to virus." in: The Journal of biological chemistry, Vol. 289, Issue 1, pp. 299-311, (2014) (PubMed).
: "In vitro inhibition of vesicular stomatitis virus replication by purified porcine Mx1 protein fused to HIV-1 Tat protein transduction domain (PTD)." in: Antiviral research, Vol. 99, Issue 2, pp. 149-57, (2013) (PubMed).
: "Neuropilin-1 biases dendrite polarization in the retina." in: Development (Cambridge, England), Vol. 140, Issue 14, pp. 2933-41, (2013) (PubMed).
: "Surface display of rice stripe virus NSvc2 and analysis of its membrane fusion activity." in: Virologica Sinica, Vol. 27, Issue 2, pp. 100-8, (2012) (PubMed).
: "Engineering fibrinogen-binding VSV-G envelope for spatially- and cell-controlled lentivirus delivery through fibrin hydrogels." in: Biomaterials, Vol. 32, Issue 12, pp. 3330-9, (2011) (PubMed).
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: "Mitogen-activated protein kinase-mediated licensing of interferon regulatory factor 3/7 reinforces the cell response to virus." in: The Journal of biological chemistry, Vol. 289, Issue 1, pp. 299-311, (2014) (PubMed).
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- 抗原
- VSV-g Tag (VSV-G Epitope Tag)
- 别名
- VSV-G-Tag (VSV-g Tag 产品)
- 物质类
- Tag
- 背景
- Rabbit Anti-VSV-G-tag Polyclonal Antibody is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% Sodium azide. It is purified by immunoaffinity chromatography.
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