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DYKDDDDK Tag 抗体

Name: DYKDDDDK Tag 抗体
Brand: antibodies-online
SKU: ABIN1573877
Availability: OutOfStock

This anti-DYKDDDDK Tag antibody is a 兔多克隆 antibody detecting DYKDDDDK Tag in WB, ELISA, IF 和 FACS. Suitable for . This Primary Antibody has been cited in 9+ publications.

Western blot analysis of DYKDDDDK-BAP control fusion protein (MW~ 49 kDa) using 1 µg/mL Rabbit Anti-DYKDDDDK-tag Polyclonal Antibody (ABIN398402) Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (ABIN398323, 1: 20,000) The signal was developed with LumiSensorTM HRP Substrate Kit (ABIN769939) (DYKDDDDK Tag 抗体)

Western blot analysis of DYKDDDDK fusion protein (MW~ 49 kDa) using 1 µg/mL Rabbit Anti-DYKDDDDK-tag Polyclonal Antibody (ABIN398402) Lane 1-2: DYKDDDDK-tag fusion protein expressed in E. (DYKDDDDK Tag 抗体)

Western blot analysis of DYKDDDDK tagged fusion proteins expressed in E. (DYKDDDDK Tag 抗体)

3 images

(9 references)

产品编号 ABIN1573877

发货至: 中国

Datasheet as PDF

Quick Overview for DYKDDDDK Tag 抗体 (ABIN1573877)

抗原

DYKDDDDK Tag

适用

请咨询

宿主

兔

克隆类型

多克隆

标记

This DYKDDDDK Tag antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

序列

DYKDDDDK

特异性

This Antibody recognizes C-terminal, N-terminal, and internal tagged fusion proteins.

纯化方法

Immunoaffinity chromatography

免疫原

A synthetic peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated - KLH

亚型

IgG
anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal FG4R unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF 宿主: 小鼠 unconjugated

anti-DYKDDDDK Tag antibody
适用: 标记 WB, ELISA, IP, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2F11G1 unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF, IC 宿主: 小鼠 Monoclonal unconjugated

anti-DYKDDDDK Tag antibody
适用: 人 WB 宿主: 兔 Polyclonal RB0493 unconjugated

anti-DYKDDDDK Tag antibody (HRP)
WB, ELISA, IF 宿主: 小鼠 Monoclonal 5A8E5 HRP

anti-DYKDDDDK Tag antibody
WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF 宿主: 小鼠 Monoclonal F-tag-01 unconjugated

应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.01-0.05 µg/mL
Western blot: 0.1-1.0 µg/mLWestern Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Immunofluorescence:5-20 µg/mLFlow Cytometry:1-3 µg for 1 x 106 cellsOther applications: user-optimized

限制

仅限研究用
状态

Lyophilized

浓度

0.5 mg/mL

缓冲液

PBS, pH 7.4, containing 0.02 % Sodium azide

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

储存条件

4 °C/-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Wang, Liu, Ye, Liu, Wu, Huang, Shi: "Ddx56 maintains proliferation of mouse embryonic stem cells via ribosome assembly and interaction with the Oct4/Sox2 complex." in: Stem cell research & therapy, Vol. 11, Issue 1, pp. 314, (2020) (PubMed).

Hoshii, Cifani, Feng, Huang, Koche, Chen, Delaney, Lowe, Kentsis, Armstrong: "A Non-catalytic Function of SETD1A Regulates Cyclin K and the DNA Damage Response." in: Cell, Vol. 172, Issue 5, pp. 1007-1021.e17, (2019) (PubMed).

Li, Liu, Feng, Wang, Das, Xu, Zhou, Sun, Guan, Xiong, Lei: "Glyceraldehyde-3-phosphate dehydrogenase is activated by lysine 254 acetylation in response to glucose signal." in: The Journal of biological chemistry, Vol. 289, Issue 6, pp. 3775-85, (2014) (PubMed).

Huang, Lv, Liu, Zha, Zhang, Jiang, Xiong, Lei, Guan: "The N-terminal phosphodegron targets TAZ/WWTR1 protein for SCF?-TrCP-dependent degradation in response to phosphatidylinositol 3-kinase inhibition." in: The Journal of biological chemistry, Vol. 287, Issue 31, pp. 26245-53, (2012) (PubMed).

Liu, Lv, Li, Xu, Zhou, Zhao, Xiong, Lei, Guan: "PP1 cooperates with ASPP2 to dephosphorylate and activate TAZ." in: The Journal of biological chemistry, Vol. 286, Issue 7, pp. 5558-66, (2011) (PubMed).

Zhou, Li, Feng, Hsiang, Xiong, Guan, Lei: "Targeted polyubiquitylation of RASSF1C by the Mule and SCF?-TrCP ligases in response to DNA damage." in: The Biochemical journal, Vol. 441, Issue 1, pp. 227-36, (2011) (PubMed).

Bedoya, Martínez, Rubio, Daròs: "Simultaneous equimolar expression of multiple proteins in plants from a disarmed potyvirus vector." in: Journal of biotechnology, Vol. 150, Issue 2, pp. 268-75, (2010) (PubMed).

Liu, Zha, Zhou, Zhang, Huang, Zhao, Li, Chan, Lim, Hong, Zhao, Xiong, Lei, Guan: "The hippo tumor pathway promotes TAZ degradation by phosphorylating a phosphodegron and recruiting the SCF{beta}-TrCP E3 ligase." in: The Journal of biological chemistry, Vol. 285, Issue 48, pp. 37159-69, (2010) (PubMed).

Zhang, Liu, Zha, Zhao, Yao, Zhao, Xiong, Lei, Guan: "TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition." in: The Journal of biological chemistry, Vol. 284, Issue 20, pp. 13355-62, (2009) (PubMed).
抗原

DYKDDDDK Tag

别名

DYKDDDDK-Tag

物质类

Tag

背景

Rabbit Anti-DYKDDDDK-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% Sodium azide.