EIF3F 抗体 (AA 81-130)
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北京 101111
Quick Overview for EIF3F 抗体 (AA 81-130) (ABIN1534899)
抗原
See all EIF3F 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 81-130
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原理
- eIF3ε Antibody
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特异性
- EIF3ε Polyclonal Antibody detects endogenous levels of eIF3ε protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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纯度
- > 95 %
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免疫原
- The antiserum was produced against synthesized peptide derived from human EIF3F. AA range:81-130
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亚型
- IgG
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anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) antibody
EIF3F 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 1-357) antibodyEIF3F 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 88-357) antibodyEIF3F 适用: 人 IHC, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (Internal Region) antibodyEIF3F 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 88-357) antibodyEIF3F 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 258-357) antibodyEIF3F 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 279-328) antibodyEIF3F 适用: 人, 小鼠, 大鼠, Cow, 猴, 兔, 犬, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 97-146) antibodyEIF3F 适用: 人, 小鼠, 大鼠, Cow, 猴, 兔, 马, 豚鼠, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 307-357) antibodyEIF3F 适用: 人, 小鼠 WB, IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (Internal Region) antibodyEIF3F 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C/1 year
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有效期
- 12 months
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- EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))
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别名
- EIF3F
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背景
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Synonyms: EIF3F, EIF3S5, Eukaryotic translation initiation factor 3 subunit F, eIF3f, Deubiquitinating enzyme eIF3f, Eukaryotic translation initiation factor 3 subunit 5, eIF-3-epsilon, eIF3 p47
Background: function:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.,mass spectrometry: PubMed:17322308,mass spectrometry: PubMed:18599441,PTM:Phosphorylated. Phosphorylation is enhanced upon serum stimulation.,similarity:Belongs to the eIF-3 subunit F family.,similarity:Contains 1 MPN (JAB/Mov34) domain.,subunit:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I, module B is composed of EIF3F, EIF3H, and EIF3M, and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of FRAP1 and RAPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.,
Gene Name: EIF3F
Protein Name: Eukaryotic translation initiation factor 3 subunit F
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分子量
- 37 kDa
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基因ID
- 8665
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OMIM
- 603914
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UniProt
- O00303
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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