CDC27 抗体 (AA 361-410)
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Quick Overview for CDC27 抗体 (AA 361-410) (ABIN1534318)
抗原
See all CDC27 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 361-410
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原理
- Cdc27 Antibody
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特异性
- Cdc27 Polyclonal Antibody detects endogenous levels of Cdc27 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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纯度
- > 95 %
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免疫原
- The antiserum was produced against synthesized peptide derived from human H-NUC. AA range:361-410
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亚型
- IgG
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应用备注
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C/1 year
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有效期
- 12 months
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- CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))
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别名
- H-NUC
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背景
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Synonyms: CDC27, ANAPC3, D0S1430E, D17S978E, Cell division cycle protein 27 homolog, Anaphase-promoting complex subunit 3, APC3, CDC27 homolog, CDC27Hs, H-NUC
Background: The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins.The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been i
Gene Name: CDC27
Protein Name: Cell division cycle protein 27 homolog
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分子量
- 91 kDa
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基因ID
- 996
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OMIM
- 116946
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UniProt
- P30260
抗原
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