EID1 抗体 (AA 71-120)
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Quick Overview for EID1 抗体 (AA 71-120) (ABIN1534225)
抗原
See all EID1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 71-120
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原理
- EID-1 Antibody
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特异性
- EID-1 Polyclonal Antibody detects endogenous levels of EID-1 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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纯度
- > 95 %
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免疫原
- The antiserum was produced against synthesized peptide derived from human EID1. AA range:71-120
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亚型
- IgG
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anti-EP300 Interacting Inhibitor of Differentiation 1 (EID1) (AA 1-187) antibody
EID1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-EP300 Interacting Inhibitor of Differentiation 1 (EID1) (Internal Region) antibodyEID1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-EP300 Interacting Inhibitor of Differentiation 1 (EID1) (AA 116-187) antibodyEID1 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-EP300 Interacting Inhibitor of Differentiation 1 (EID1) (Center) antibodyEID1 适用: 人, Cow WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-EP300 Interacting Inhibitor of Differentiation 1 (EID1) (AA 1-19) antibodyEID1 适用: 人 ELISA, WB, IF 宿主: 小鼠 Monoclonal 2 unconjugated
anti-EP300 Interacting Inhibitor of Differentiation 1 (EID1) (AA 159-187) antibodyEID1 适用: 人 ELISA, WB, IF 宿主: 小鼠 Monoclonal 26 unconjugated
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应用备注
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C/1 year
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有效期
- 12 months
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- EID1 (EP300 Interacting Inhibitor of Differentiation 1 (EID1))
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别名
- EID1
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背景
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Synonyms: EID1, C15orf3, CRI1, RBP21, PNAS-22, PTD014, EP300-interacting inhibitor of differentiation 1, 21 kDa pRb-associated protein, CREBBP/EP300 inhibitory protein 1, E1A-like inhibitor of differentiation 1, EID-1
Background: developmental stage:Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level).,function:Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.,induction:Down-regulated in differentiating U937 leukemia cells.,miscellaneous:Inhibition of MYOD1 may be partly due to the ability of EID1 to bind and inhibit EP300 histone acetyltransferase activity.,PTM:Ubiquitinated in U-2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.,subcellular location:May shuttle between nucleus and cytoplasm.,subunit:Interacts via its LXCXE motif with the entire pocket region of RB1. Interacts with EP300, NR0B2 and TRIM27.,tissue specificity:Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A549 and various leukemia cell lines.,
Gene Name: EID1
Protein Name: EP300-interacting inhibitor of differentiation 1
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分子量
- 20 kDa
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基因ID
- 23741
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OMIM
- 605894
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UniProt
- Q9Y6B2
抗原
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