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Vinculin 抗体 (AA 786-835)

The 兔 多克隆 anti-Vinculin antibody is suitable to detect Vinculin in samples from 人, 小鼠 和 大鼠. It has been validated for WB, IHC, ELISA 和 IF.
产品编号 ABIN1532662
发货至: 中国
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Quick Overview for Vinculin 抗体 (AA 786-835) (ABIN1532662)

抗原

See all Vinculin (VCL) 抗体
Vinculin (VCL)

适用

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人, 小鼠, 大鼠

宿主

  • 153
  • 66
兔

克隆类型

  • 119
  • 100
多克隆

标记

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This Vinculin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
  • 抗原表位

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    AA 786-835

    原理

    Vinculin Antibody

    特异性

    Vinculin Polyclonal Antibody detects endogenous levels of Vinculin protein.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    纯度

    > 95 %

    免疫原

    The antiserum was produced against synthesized peptide derived from human Vinculin. AA range:786-835

    亚型

    IgG
  • 应用备注

    WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20°C/1 year

    有效期

    12 months
  • 抗原

    Vinculin (VCL)

    别名

    Vinculin

    背景

    Synonyms: VCL, Vinculin, Metavinculin

    Background: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008],

    Gene Name: VCL

    Protein Name: Vinculin

    分子量

    123 kDa

    基因ID

    7414

    OMIM

    193065

    UniProt

    P18206

    途径

    Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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