MLK1/2 抗体 (pThr266, pThr312)
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北京 101111
Quick Overview for MLK1/2 抗体 (pThr266, pThr312) (ABIN1532062)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 281-330, pThr266, pThr312
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原理
- MLK1/2 (phospho Thr312/266) Antibody
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特异性
- Phospho-MLK1/2 (T312/266) Polyclonal Antibody detects endogenous levels of MLK1/2 protein only when phosphorylated at T312/266.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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纯度
- > 95 %
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免疫原
- The antiserum was produced against synthesized peptide derived from human MLK1/2 around the phosphorylation site of Thr312/266. AA range:281-330
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亚型
- IgG
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应用备注
- IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C/1 year
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有效期
- 12 months
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- MLK1/2
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背景
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Synonyms: MAP3K9, MLK1, PRKE1, Mitogen-activated protein kinase kinase kinase 9, Mixed lineage kinase 1, MAP3K10, MLK2, MST, Mitogen-activated protein kinase kinase kinase 10, Mixed lineage kinase 2, Protein kinase MST
Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.,function:Activates the JUN N-terminal pathway.,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH3 domain.,subunit:Homodimer.,tissue specificity:Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.,
Gene Name: MAP3K9/MAP3K10
Protein Name: Mitogen-activated protein kinase kinase kinase 9/10
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分子量
- 121 kDa
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基因ID
- 4293, 4294
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OMIM
- 600136
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UniProt
- P80192
抗原
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