CRYAB 抗体 (pSer19)
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Quick Overview for CRYAB 抗体 (pSer19) (ABIN1531572)
抗原
See all CRYAB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 10-59, pSer19
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原理
- Crystallin-αB (phospho Ser19) Antibody
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特异性
- Phospho-Crystallin-αB (S19) Polyclonal Antibody detects endogenous levels of Crystallin-αB protein only when phosphorylated at S19.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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纯度
- > 95 %
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免疫原
- The antiserum was produced against synthesized peptide derived from human CRYAB around the phosphorylation site of Ser19. AA range:10-59
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亚型
- IgG
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应用备注
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C/1 year
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有效期
- 12 months
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- CRYAB (Crystallin, alpha B (CRYAB))
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别名
- CRYAB
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背景
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Synonyms: CRYAB, CRYA2, Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component
Background: Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone, instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits, the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distin
Gene Name: CRYAB
Protein Name: Alpha-crystallin B chain
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分子量
- 20 kDa
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基因ID
- 1410
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OMIM
- 123590
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UniProt
- P02511
抗原
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